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Original scientific paper
https://doi.org/10.5562/cca2146

Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA

Vlatka Godinić-Mikulčić   ORCID icon orcid.org/0000-0003-0319-8035 ; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia
Jelena Jarić ; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia
Ivana Weygand-Đurašević ; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia

Fulltext: english, pdf (6 MB) pages 441-449 downloads: 726* cite
APA 6th Edition
Godinić-Mikulčić, V., Jarić, J. & Weygand-Đurašević, I. (2012). Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA. Croatica Chemica Acta, 85 (4), 441-449. https://doi.org/10.5562/cca2146
MLA 8th Edition
Godinić-Mikulčić, Vlatka, et al. "Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA." Croatica Chemica Acta, vol. 85, no. 4, 2012, pp. 441-449. https://doi.org/10.5562/cca2146. Accessed 25 Sep. 2021.
Chicago 17th Edition
Godinić-Mikulčić, Vlatka, Jelena Jarić and Ivana Weygand-Đurašević. "Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA." Croatica Chemica Acta 85, no. 4 (2012): 441-449. https://doi.org/10.5562/cca2146
Harvard
Godinić-Mikulčić, V., Jarić, J., and Weygand-Đurašević, I. (2012). 'Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA', Croatica Chemica Acta, 85(4), pp. 441-449. https://doi.org/10.5562/cca2146
Vancouver
Godinić-Mikulčić V, Jarić J, Weygand-Đurašević I. Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA. Croatica Chemica Acta [Internet]. 2012 [cited 2021 September 25];85(4):441-449. https://doi.org/10.5562/cca2146
IEEE
V. Godinić-Mikulčić, J. Jarić and I. Weygand-Đurašević, "Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA", Croatica Chemica Acta, vol.85, no. 4, pp. 441-449, 2012. [Online]. https://doi.org/10.5562/cca2146

Abstracts
Several studies have revealed the involvement of multi aminoacyl-tRNA synthetase complexes (MSC) in archaeal and eukaryotic translation. Here we analyzed interactions of atypical Methanothermobacter thermautotrophicus seryl-tRNA synthetase (MtSerRS), transfer RNA (tRNASer) and arginyl-tRNA synthetase (ArgRS). Surface plasmon resonance (SPR) was used to determine dissociation constants for the MtSerRS:tRNASer complex and the results were consistent with cooperative binding of tRNASer. This finding was supported by the ability of MtSerRS to bind two tRNAs in gel mobility shift assay. Notably, the MtSerRS:tRNASer complex formation was stimulated by MtArgRS, previously determined interacting partner of MtSerRS. MtArgRS decreases Kd for MtSerRS:tRNASer two-fold, but does not affect cooperative properties or stoichiometry of the complex. Further investigation of complex formation between MtSerRS and tRNASer showed that this molecular interaction is salt-dependent. The most pronounced improvements in binding were determined at high ionic strength, using Tris as a buffering agent, while the addition of Mg2+ ions led to the same SPR response. (doi: 10.5562/cca2146)

Keywords
aminoacyl-tRNA synthetase; seryl-tRNA synthetase; arginyl-tRNA synthetase; tRNA; surface plasmon resonance

Hrčak ID: 93732

URI
https://hrcak.srce.hr/93732

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