Stručni rad
SITE-DIRECTED MUTAGENESIS AND GENETIC MARKERS IN SELECTION OF PIGS
Milanko Stupar
; Faculty of Agriculture, Novi Sad, Serbia
Vitomir Vidović
; Faculty of Agriculture, Novi Sad, Serbia
Dragomir Lukač
; Faculty of Agriculture, Novi Sad, Serbia
Ljuba Štrbac
; Faculty of Agriculture, Novi Sad, Serbia
Sažetak
The idea behind marker assisted selection is that there are genes with significant effects that may be targeted specifically in selection. Some traits are controled by single genes. Those genes may be engineered to produce proteins that are tailored for a specific application. Site-directed mutagenesis (SDM) is used to generate mutations that can produce rationally designed protein that has improved special properties. Gene targeting using SDM has been combined with somatic cell nuclear transfer and germ cell transplantation to generate gene-targeted animal models. Targeted mutagenesis can be used to incorporated point mutation, insertion or deletion into coding ragion of a chosen gene in the epitope region. Also, promoter activity can be regulated by SDM to influence expression level of the targeting gene. SDM with gene targeting based on homologous recombination is the ultimate mutation breeding technology because it enables useful information acquired from structural-based protein engineering, to be applied directly to molecular breeding of pigs.
Ključne riječi
molecular breeding; selection; site-directed mutagenesis
Hrčak ID:
97643
URI
Datum izdavanja:
4.2.2013.
Posjeta: 1.348 *