Acta Pharmaceutica, Vol. 63 No. 2, 2013.
Kratko priopćenje
https://doi.org/10.2478/acph-2013-0017
Preparation and application of a molecularly imprinted polymer for determination of glibenclamide residues
RYM LAHSINI
; Laboratoire de Chimie Analytique et d’Electrochimie, Université de Carthage, Institut National des Sciences Appliquées et de Technologie, 1080 Tunis Cedex, Tunisia
MOHAMED R. LOUHAICHI
; Laboratoire National de Contrôle des Médicaments, 1006 Tunis, Tunisia
NAFAA ADHOUM
; Laboratoire de Chimie Analytique et d’Electrochimie, Université de Carthage, Institut National des Sciences Appliquées et de Technologie, 1080 Tunis Cedex, Tunisia
LOTFI MONSER
; Laboratoire de Chimie Analytique et d’Electrochimie, Université de Carthage, Institut National des Sciences Appliquées et de Technologie, 1080 Tunis Cedex, Tunisia
Sažetak
The performance of a molecularly imprinted polymer (MIP) as selective packing material for solid-phase extraction (SPE) of residual glibenclamide in an industrial process was investigated. MIP was prepared using 4-vinylpyridine as monomer, ethylenedimethacrylate as cross linker, 2,2’-azobis-2-methylpropionitrile as initiator and dimethyl formamide as porogen. Use of acetonitrile as a rebinding solvent allows good recognition of the glibenclamide template. It was found that this polymer can be used for determination of trace levels of glibenclamide with a recovery percentage that could reach 87.1 %. Furthermore, the synthesized MIP showed higher selectivity towards glibenclamide than other compounds such as glimepiride and metformine. The synthesized MIP enabled direct determination of the target contaminant after an enrichment step that allowed quantification of a low glibenclamide concentration as low as 0.016 mg L–1. Combination of high performance liquid chromatography with MIP-SPE could be successfully used for quality control of pharmaceuticals during the cleaning process in the production of dry drug forms.
Ključne riječi
glibenclamide; molecular imprinted polymer; solid phase extraction; HPLC
Hrčak ID:
97703
URI
Datum izdavanja:
30.6.2013.
Posjeta: 2.358 *