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https://doi.org/10.2478/acph-2013-0020

Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification

AJIT A. PATIL ; Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004, India
SACHIN S. BHUSARI ; Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004, India
DEVANAND B. SHINDE ; Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004, India
PRAVIN S. WAKTE ; Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004, India

Puni tekst: engleski, pdf (201 KB) str. 193-207 preuzimanja: 1.137* citiraj
APA 6th Edition
PATIL, A.A., BHUSARI, S.S., SHINDE, D.B. i WAKTE, P.S. (2013). Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification. Acta Pharmaceutica, 63 (2), 193-207. https://doi.org/10.2478/acph-2013-0020
MLA 8th Edition
PATIL, AJIT A., et al. "Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification." Acta Pharmaceutica, vol. 63, br. 2, 2013, str. 193-207. https://doi.org/10.2478/acph-2013-0020. Citirano 25.06.2019.
Chicago 17th Edition
PATIL, AJIT A., SACHIN S. BHUSARI, DEVANAND B. SHINDE i PRAVIN S. WAKTE. "Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification." Acta Pharmaceutica 63, br. 2 (2013): 193-207. https://doi.org/10.2478/acph-2013-0020
Harvard
PATIL, A.A., et al. (2013). 'Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification', Acta Pharmaceutica, 63(2), str. 193-207. doi: https://doi.org/10.2478/acph-2013-0020
Vancouver
PATIL AA, BHUSARI SS, SHINDE DB, WAKTE PS. Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification. Acta Pharm. [Internet]. 2013 [pristupljeno 25.06.2019.];63(2):193-207. doi: https://doi.org/10.2478/acph-2013-0020
IEEE
A.A. PATIL, S.S. BHUSARI, D.B. SHINDE i P.S. WAKTE, "Optimization of process variables for phyllanthin extraction from Phyllanthus amarus leaves by supercritical fluid using a Box-Behnken experimental design followed by HPLC identification", Acta Pharmaceutica, vol.63, br. 2, str. 193-207, 2013. [Online]. doi: https://doi.org/10.2478/acph-2013-0020

Sažetak
The response surface methodology using the Box-Behnken design was established to describe supercritical carbon dioxide assisted extraction of phyllanthin from Phyllanthus amarus Schum and Thonn leaves prior to HPLC analysis. The effects of extraction pressure, temperature, modifier concentration and extraction time on the yield of phyllanthin were investigated. By solving the regression equation, the optimum conditions were as follows: extraction pressure 23.2 MPa, temperature 40 °C, methanol as modifier at a concentration 10 % and time 90 min. Under these conditions, the phyllanthin yield was 12.83 ± 0.28 mg g-1, which was in good agreement with the predicted values. Modifier concentration and extraction time showed a significant effect on the phyllanthin yield.

Ključne riječi
phyllanthin; Phyllanthus amarus (Euphorbiaceae); supercritical carbon dioxide assisted extraction; Box-Behnken design; HPLC

Hrčak ID: 97697

URI
https://hrcak.srce.hr/97697

Posjeta: 1.321 *