Prethodno priopćenje
Detection of cathepsin D in ewe’s milk by Western Blotting method
Iva Dolenčić Špehar
orcid.org/0000-0002-8036-583X
; University of Zagreb, Faculty of Agriculture, Dairy Science Department, Svetošimunska 25, 10000 Zagreb, Croatia
Franjo Martinković
Jasmina Havranek
Albert Marinculić
Milna Tudor Kalit
Samir Kalit
orcid.org/0000-0003-1460-2072
; University of Zagreb, Faculty of Agriculture, Dairy Science Department, Svetošimunska 25, 10000 Zagreb, Croatia
Sažetak
Milk contains about 70 indogene enzymes, while only twenty of them were investigated. One of the less explored is the milk enzyme cathepsin D, proteolytic enzyme located in the lysosomes, which are an integral part of the somatic cells whose number varies depending on the animal’s health. Unlike cow’s milk, in ewe’s milk the presence of cathepsin D was not determined, which could affect the production of dairy products, especially cheese, which are traditionally produced in Croatia and Mediterranean. This paper shows the presence of cathepsin D and its forms in ewe’s milk by modified Western Blotting method. The analysis confirmed the presence of procathepsin D, mature and heavy chain of cathepsin D. Pseudocathepsin D and light chain of cathepsin D were not detected.
Ključne riječi
milk; cathepsin D; ewe’s milk; Western Blotting
Hrčak ID:
98228
URI
Datum izdavanja:
13.3.2013.
Posjeta: 1.878 *