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Izvorni znanstveni članak
Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens
Sanja Šegović
; Zavod za bolesti zubi Stomatološki fakultet Gundulićeva 5, Zagreb, Hrvatska
Dunja Buntak-Kobler
; Zavod za bolesti zubi Stomatološki fakultet Gundulićeva 5, Zagreb, Hrvatska
Albert Marinculić
; Odjel za parazitologiju Veterinarski fakultet, Heinzelova 55, Zagreb, Hrvatska
Jasminka Granić
; Laboratorij za kliničku parazitologiju Klinička bolnica za infektivne bolesti “Dr. Fran Mihaljević”, Rockfellerova 3, Zagreb, Hrvatska
Dora Najžar-Fleger
; Zavod za bolesti zubi Stomatološki fakultet Gundulićeva 5, Zagreb, Hrvatska
Emir Duraković
; Odjel za parazitologiju Veterinarski fakultet, Heinzelova 55, Zagreb, Hrvatska
| Puni tekst: engleski, pdf (10 MB) | str. 567-575 | citiraj | |
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APA 6th Edition Šegović, S., Buntak-Kobler, D., Marinculić, A., Granić, J., Najžar-Fleger, D. i Duraković, E. (1998). Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens. Acta stomatologica Croatica, 32 (4), 567-575. Preuzeto s https://hrcak.srce.hr/98250 MLA 8th Edition Šegović, Sanja, et al. "Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens." Acta stomatologica Croatica, vol. 32, br. 4, 1998, str. 567-575. https://hrcak.srce.hr/98250. Citirano 04.03.2021. Chicago 17th Edition Šegović, Sanja, Dunja Buntak-Kobler, Albert Marinculić, Jasminka Granić, Dora Najžar-Fleger i Emir Duraković. "Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens." Acta stomatologica Croatica 32, br. 4 (1998): 567-575. https://hrcak.srce.hr/98250 Harvard Šegović, S., et al. (1998). 'Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens', Acta stomatologica Croatica, 32(4), str. 567-575. Preuzeto s: https://hrcak.srce.hr/98250 (Datum pristupa: 04.03.2021.) Vancouver Šegović S, Buntak-Kobler D, Marinculić A, Granić J, Najžar-Fleger D, Duraković E. Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens. Acta stomatologica Croatica [Internet]. 1998 [pristupljeno 04.03.2021.];32(4):567-575. Dostupno na: https://hrcak.srce.hr/98250 IEEE S. Šegović, D. Buntak-Kobler, A. Marinculić, J. Granić, D. Najžar-Fleger i E. Duraković, "Proteolytic Activity of Trichomonas tenax from Dentobacterial Plaque and Root Canal Contens", Acta stomatologica Croatica, vol.32, br. 4, str. 567-575, 1998. [Online]. Dostupno na: https://hrcak.srce.hr/98250. [Citirano: 04.03.2021.] |
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| Puni tekst: hrvatski, pdf (10 MB) | str. 567-575 | citiraj | |
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APA 6th Edition Šegović, S., Buntak-Kobler, D., Marinculić, A., Granić, J., Najžar-Fleger, D. i Duraković, E. (1998). Proteolitička aktivnost oralnoga protozoona Trichomonas tenax iz zubnoga plaka i sadržaja korijenskih kanala. Acta stomatologica Croatica, 32 (4), 567-575. Preuzeto s https://hrcak.srce.hr/98250 MLA 8th Edition Šegović, Sanja, et al. "Proteolitička aktivnost oralnoga protozoona Trichomonas tenax iz zubnoga plaka i sadržaja korijenskih kanala." Acta stomatologica Croatica, vol. 32, br. 4, 1998, str. 567-575. https://hrcak.srce.hr/98250. Citirano 04.03.2021. Chicago 17th Edition Šegović, Sanja, Dunja Buntak-Kobler, Albert Marinculić, Jasminka Granić, Dora Najžar-Fleger i Emir Duraković. "Proteolitička aktivnost oralnoga protozoona Trichomonas tenax iz zubnoga plaka i sadržaja korijenskih kanala." Acta stomatologica Croatica 32, br. 4 (1998): 567-575. https://hrcak.srce.hr/98250 Harvard Šegović, S., et al. (1998). 'Proteolitička aktivnost oralnoga protozoona Trichomonas tenax iz zubnoga plaka i sadržaja korijenskih kanala', Acta stomatologica Croatica, 32(4), str. 567-575. Preuzeto s: https://hrcak.srce.hr/98250 (Datum pristupa: 04.03.2021.) Vancouver Šegović S, Buntak-Kobler D, Marinculić A, Granić J, Najžar-Fleger D, Duraković E. Proteolitička aktivnost oralnoga protozoona Trichomonas tenax iz zubnoga plaka i sadržaja korijenskih kanala. Acta stomatologica Croatica [Internet]. 1998 [pristupljeno 04.03.2021.];32(4):567-575. Dostupno na: https://hrcak.srce.hr/98250 IEEE S. Šegović, D. Buntak-Kobler, A. Marinculić, J. Granić, D. Najžar-Fleger i E. Duraković, "Proteolitička aktivnost oralnoga protozoona Trichomonas tenax iz zubnoga plaka i sadržaja korijenskih kanala", Acta stomatologica Croatica, vol.32, br. 4, str. 567-575, 1998. [Online]. Dostupno na: https://hrcak.srce.hr/98250. [Citirano: 04.03.2021.] |
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Sažetak
The purpose of this study was to examine proteolytic activity of Trichomonas tenax collected directly from dentobacterial plaque and root canal contents o f gangrenous teeth or teeth with periapical processes. Both electrophoretic method involving polyacrylamide gels and electrophoretic method involving gelatin - containing polyacrylamide gels, have been used to analyse Trichomonas tenax proteolytic activity. The most obvious and fastest activities were obtained when gels were incubated in pH 4.6, followed by the results of incubating in pH 5.6, while in pH 2.8 activity was less but still obvious. Proteolytic activities were the most effective in the area of protein MW 36 kDa. The different activity of enzymes depending on the pH o f incubated media indicate the presence of different endopeptidases in cell lysates of protozoon Trichomonas tenax from dentobacterial plaque and root canal contents.
Ključne riječi
Trichomonas tenax; proteolytic activity; dentobacterial plaque; root canal
Hrčak ID: 98250
URI
https://hrcak.srce.hr/98250
Moj profil
Srce