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https://doi.org/10.15644/asc47/1/2

The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study

Ana Badovinac ; Department of Periodontology, School of Dental Medicine, Zagreb, and Clinical Department of Periodontology, Clinical Hospital Center
Genadij Razdorov ; Center for Translational and Clinical Research, School of Medicine, Zagreb, Croatia
Lovorka Grgurević ; Center for Translational and Clinical Research, School of Medicine, Zagreb, Croatia
Ivan Puhar ; Department of Periodontology, School of Dental Medicine, Zagreb, and Clinical Department of Periodontology, Clinical Hospital Center
Darije Plančak ; Department of Periodontology, School of Dental Medicine, Zagreb, and Clinical Department of Periodontology, Clinical Hospital Center
Darko Božić ; Department of Periodontology, School of Dental Medicine, Zagreb, and Clinical Department of Periodontology, Clinical Hospital Center

Puni tekst: engleski, pdf (496 KB) str. 10-20 preuzimanja: 332* citiraj
APA 6th Edition
Badovinac, A., Razdorov, G., Grgurević, L., Puhar, I., Plančak, D. i Božić, D. (2013). The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study. Acta stomatologica Croatica, 47 (1), 10-20. https://doi.org/10.15644/asc47/1/2
MLA 8th Edition
Badovinac, Ana, et al. "The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study." Acta stomatologica Croatica, vol. 47, br. 1, 2013, str. 10-20. https://doi.org/10.15644/asc47/1/2. Citirano 02.03.2021.
Chicago 17th Edition
Badovinac, Ana, Genadij Razdorov, Lovorka Grgurević, Ivan Puhar, Darije Plančak i Darko Božić. "The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study." Acta stomatologica Croatica 47, br. 1 (2013): 10-20. https://doi.org/10.15644/asc47/1/2
Harvard
Badovinac, A., et al. (2013). 'The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study', Acta stomatologica Croatica, 47(1), str. 10-20. https://doi.org/10.15644/asc47/1/2
Vancouver
Badovinac A, Razdorov G, Grgurević L, Puhar I, Plančak D, Božić D. The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study. Acta stomatologica Croatica [Internet]. 2013 [pristupljeno 02.03.2021.];47(1):10-20. https://doi.org/10.15644/asc47/1/2
IEEE
A. Badovinac, G. Razdorov, L. Grgurević, I. Puhar, D. Plančak i D. Božić, "The Application of LC-MS/MS Technology for Proteomic Analysis of Gingival Tissue: a Pilot Study", Acta stomatologica Croatica, vol.47, br. 1, str. 10-20, 2013. [Online]. https://doi.org/10.15644/asc47/1/2
Puni tekst: hrvatski, pdf (496 KB) str. 10-20 preuzimanja: 429* citiraj
APA 6th Edition
Badovinac, A., Razdorov, G., Grgurević, L., Puhar, I., Plančak, D. i Božić, D. (2013). Primjena tehnologije LC-MS/MS za proteomsku analizu gingivnog tkiva: eksperimentalno istraživanje. Acta stomatologica Croatica, 47 (1), 10-20. https://doi.org/10.15644/asc47/1/2
MLA 8th Edition
Badovinac, Ana, et al. "Primjena tehnologije LC-MS/MS za proteomsku analizu gingivnog tkiva: eksperimentalno istraživanje." Acta stomatologica Croatica, vol. 47, br. 1, 2013, str. 10-20. https://doi.org/10.15644/asc47/1/2. Citirano 02.03.2021.
Chicago 17th Edition
Badovinac, Ana, Genadij Razdorov, Lovorka Grgurević, Ivan Puhar, Darije Plančak i Darko Božić. "Primjena tehnologije LC-MS/MS za proteomsku analizu gingivnog tkiva: eksperimentalno istraživanje." Acta stomatologica Croatica 47, br. 1 (2013): 10-20. https://doi.org/10.15644/asc47/1/2
Harvard
Badovinac, A., et al. (2013). 'Primjena tehnologije LC-MS/MS za proteomsku analizu gingivnog tkiva: eksperimentalno istraživanje', Acta stomatologica Croatica, 47(1), str. 10-20. https://doi.org/10.15644/asc47/1/2
Vancouver
Badovinac A, Razdorov G, Grgurević L, Puhar I, Plančak D, Božić D. Primjena tehnologije LC-MS/MS za proteomsku analizu gingivnog tkiva: eksperimentalno istraživanje. Acta stomatologica Croatica [Internet]. 2013 [pristupljeno 02.03.2021.];47(1):10-20. https://doi.org/10.15644/asc47/1/2
IEEE
A. Badovinac, G. Razdorov, L. Grgurević, I. Puhar, D. Plančak i D. Božić, "Primjena tehnologije LC-MS/MS za proteomsku analizu gingivnog tkiva: eksperimentalno istraživanje", Acta stomatologica Croatica, vol.47, br. 1, str. 10-20, 2013. [Online]. https://doi.org/10.15644/asc47/1/2

Sažetak
Aim: The aim of this study was to find an optimal method to identify maximal number of proteins from human gingival tissue samples in a patient with aggressive periodontitis using label-free quantitative liquid chromatography mass spectrometry technology (LC-MS/MS), and to compare proteome of healthy and diseased gingival tissue samples. Materials and methods: Four gingival tissue samples (2 healthy, 2 diseased) were obtained from a nonsmoking female patient suffering from severe generalized aggressive periodontitis. Proteins from the tissue lysate were separated by 1D gel electrophoresis, in gel digested, measured by nano-scale HPLC system coupled to a mass spectrometer through a nano-electrospray ionization source. Raw data were processed by MaxQuant software. Results: Label-free quantitative LC-MS/MS analysis together with sample preparation, 1D gel electrophoresis and in-gel digestion showed to be an effective method for the proteome analysis of the gingival periodontal tissues. A total of 2429 proteins were identified
and quantified. Among proteins upregulated in disease were: Ig gamma-1 chain C region, Ig kappa chain C region, Ig gamma-3 chain C region, and among downregulated proteins in the disease
were: Desmoplakin, annexins, S100-A8/A9 proteins, and keratins. Conclusions: This pilot study is providing a novel insight into the protein profiles of gingival healthy and diseased tissues, which may contribute to the improvements in diagnosis, prognosis and better understanding of aggressive periodontitis pathogenesis. LC-MS/MS technology proved to be an effective method for the proteome analysis of the gingival tissues.

Ključne riječi
Aggressive periodontitis; Gingiva; Proteomics; Mass Spectrometry; Biopsy; Microtubule-Associated Proteins; Immunoglobulin kappa-Chains; Antigens, CD3; Desmoplakins; Annexins; Keratins; S100 Proteins

Hrčak ID: 98646

URI
https://hrcak.srce.hr/98646

[hrvatski]

Posjeta: 1.175 *