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https://doi.org/10.5562/cca2078

Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture

Petra Peharec Štefanić ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Ana Smolko ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Anita Horvatić ; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Mario Cindrić ; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia
Marijana Krsnik-Rasol ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia
Biljana Balen   ORCID icon orcid.org/0000-0002-3745-9865 ; Department of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (4 MB) str. 27-37 preuzimanja: 697* citiraj
APA 6th Edition
Peharec Štefanić, P., Smolko, A., Horvatić, A., Cindrić, M., Krsnik-Rasol, M. i Balen, B. (2013). Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture. Croatica Chemica Acta, 86 (1), 27-37. https://doi.org/10.5562/cca2078
MLA 8th Edition
Peharec Štefanić, Petra, et al. "Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture." Croatica Chemica Acta, vol. 86, br. 1, 2013, str. 27-37. https://doi.org/10.5562/cca2078. Citirano 18.01.2020.
Chicago 17th Edition
Peharec Štefanić, Petra, Ana Smolko, Anita Horvatić, Mario Cindrić, Marijana Krsnik-Rasol i Biljana Balen. "Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture." Croatica Chemica Acta 86, br. 1 (2013): 27-37. https://doi.org/10.5562/cca2078
Harvard
Peharec Štefanić, P., et al. (2013). 'Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture', Croatica Chemica Acta, 86(1), str. 27-37. https://doi.org/10.5562/cca2078
Vancouver
Peharec Štefanić P, Smolko A, Horvatić A, Cindrić M, Krsnik-Rasol M, Balen B. Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture. Croatica Chemica Acta [Internet]. 2013 [pristupljeno 18.01.2020.];86(1):27-37. https://doi.org/10.5562/cca2078
IEEE
P. Peharec Štefanić, A. Smolko, A. Horvatić, M. Cindrić, M. Krsnik-Rasol i B. Balen, "Protein and Glycoprotein Patterns Related to Morphogenesis in Horseradish Tissue Culture", Croatica Chemica Acta, vol.86, br. 1, str. 27-37, 2013. [Online]. https://doi.org/10.5562/cca2078

Sažetak
In this study cellular proteins of in vitro grown horseradish (Armoracia lapathifolia Gilib.)
tissues of different morphologies, leaves (L), teratoma (TM) and tumor (TN), were separated by
one-dimensional (1-DE) and two-dimensional polyacrylamide gel electrophoresis (2-DE) in order to
reveal developmentally specific proteins. Moreover, proteins were subjected to analyses by lectin assays
to reveal whether the morphological differences were reflected in protein glycosylation. 1-DE glycoprotein
pattern showed substantial distinctions between horseradish tissues of different morphologies, which
was in contrast to silver stained protein profiles, where only slightly qualitative changes were observed.
2-DE revealed more differences in expression of cellular proteins between examined tissues in comparison
to 1-DE, while 2-DE blots confirmed quantitative difference in expression of 43 kDa glycoprotein
between investigated tissues. Mass spectrometry analysis identified this protein as a typical horseradish
peroxidase. Obtained results imply that the oligosaccharide structures could be important determinants of
specificities of horseradish tissue morphology. (doi: 10.5562/cca2078)

Ključne riječi
morphogenesis; horseradish; glycoproteins; lectine assay; tissue culture; 2-DE

Hrčak ID: 101905

URI
https://hrcak.srce.hr/101905

Posjeta: 996 *