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Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents

Marina Dobrivojević ; Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Ivan Bohaček ; Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Igor Erjavec ; Department of Anatomy University of Zagreb School of Medicine, Zagreb, Croatia
Dunja Gorup ; Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia
Srećko Gajović ; Croatian Institute for Brain Research, University of Zagreb School of Medicine, Zagreb, Croatia

Puni tekst: engleski, pdf (2 MB) str. 3-11 preuzimanja: 337* citiraj
APA 6th Edition
Dobrivojević, M., Bohaček, I., Erjavec, I., Gorup, D. i Gajović, S. (2013). Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents. Croatian medical journal, 54 (1), 3-11. Preuzeto s https://hrcak.srce.hr/102765
MLA 8th Edition
Dobrivojević, Marina, et al. "Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents." Croatian medical journal, vol. 54, br. 1, 2013, str. 3-11. https://hrcak.srce.hr/102765. Citirano 13.11.2019.
Chicago 17th Edition
Dobrivojević, Marina, Ivan Bohaček, Igor Erjavec, Dunja Gorup i Srećko Gajović. "Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents." Croatian medical journal 54, br. 1 (2013): 3-11. https://hrcak.srce.hr/102765
Harvard
Dobrivojević, M., et al. (2013). 'Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents', Croatian medical journal, 54(1), str. 3-11. Preuzeto s: https://hrcak.srce.hr/102765 (Datum pristupa: 13.11.2019.)
Vancouver
Dobrivojević M, Bohaček I, Erjavec I, Gorup D, Gajović S. Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents. Croat Med J. [Internet]. 2013 [pristupljeno 13.11.2019.];54(1):3-11. Dostupno na: https://hrcak.srce.hr/102765
IEEE
M. Dobrivojević, I. Bohaček, I. Erjavec, D. Gorup i S. Gajović, "Computed microtomography visualization and quantification of mouse ischemic brain lesion by nonionic radio contrast agents", Croatian medical journal, vol.54, br. 1, str. 3-11, 2013. [Online]. Dostupno na: https://hrcak.srce.hr/102765. [Citirano: 13.11.2019.]

Sažetak
Aim To explore the possibility of brain imaging by microcomputed
tomography (microCT) using x-ray contrasting
methods to visualize mouse brain ischemic lesions after
middle cerebral artery occlusion (MCAO).
Methods Isolated brains were immersed in ionic or nonionic
radio contrast agent (RCA) for 5 days and subsequently
scanned using microCT scanner. To verify whether
ex-vivo microCT brain images can be used to characterize
ischemic lesions, they were compared to Nissl stained
serial histological sections of the same brains. To verify if
brains immersed in RCA may be used afterwards for other
methods, subsequent immunofluorescent labeling with
anti-NeuN was performed.
Results Nonionic RCA showed better gray to white matter
contrast in the brain, and therefore was selected for further
studies. MicroCT measurement of ischemic lesion size and
cerebral edema significantly correlated with the values determined
by Nissl staining (ischemic lesion size: P=0.0005;
cerebral edema: P=0.0002). Brain immersion in nonionic
RCA did not affect subsequent immunofluorescent analysis
and NeuN immunoreactivity.
Conclusion MicroCT method was proven to be suitable
for delineation of the ischemic lesion from the non-infarcted
tissue, and quantification of lesion volume and cerebral
edema.

Hrčak ID: 102765

URI
https://hrcak.srce.hr/102765

Posjeta: 494 *