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Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere

Sandra Javernik Rajh ; Research & Development Division, Krka d.d., Šmarješka cesta 6, SI-8501 Novo mesto, Slovenia
Samo Kreft ; Department for Pharmacognosy, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, SI-1000 Ljubljana, Slovenia
Borut Štrukelj ; Department for Pharmacognosy, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, SI-1000 Ljubljana, Slovenia
Franc Vrečer ; Research & Development Division, Krka d.d., Šmarješka cesta 6, SI-8501 Novo mesto, Slovenia

Puni tekst: engleski, pdf (135 KB) str. 263-268 preuzimanja: 1.497* citiraj
APA 6th Edition
Javernik Rajh, S., Kreft, S., Štrukelj, B. i Vrečer, F. (2003). Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere. Croatica Chemica Acta, 76 (3), 263-268. Preuzeto s https://hrcak.srce.hr/103177
MLA 8th Edition
Javernik Rajh, Sandra, et al. "Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere." Croatica Chemica Acta, vol. 76, br. 3, 2003, str. 263-268. https://hrcak.srce.hr/103177. Citirano 27.02.2021.
Chicago 17th Edition
Javernik Rajh, Sandra, Samo Kreft, Borut Štrukelj i Franc Vrečer. "Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere." Croatica Chemica Acta 76, br. 3 (2003): 263-268. https://hrcak.srce.hr/103177
Harvard
Javernik Rajh, S., et al. (2003). 'Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere', Croatica Chemica Acta, 76(3), str. 263-268. Preuzeto s: https://hrcak.srce.hr/103177 (Datum pristupa: 27.02.2021.)
Vancouver
Javernik Rajh S, Kreft S, Štrukelj B, Vrečer F. Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere. Croatica Chemica Acta [Internet]. 2003 [pristupljeno 27.02.2021.];76(3):263-268. Dostupno na: https://hrcak.srce.hr/103177
IEEE
S. Javernik Rajh, S. Kreft, B. Štrukelj i F. Vrečer, "Comparison of CE and HPLC Methods for Determining Lovastatin and Its Oxidation Products after Exposure to an Oxidative Atmosphere", Croatica Chemica Acta, vol.76, br. 3, str. 263-268, 2003. [Online]. Dostupno na: https://hrcak.srce.hr/103177. [Citirano: 27.02.2021.]

Sažetak
Lovastatin is a cholesterol-lowering agent, which competitively inhibits the enzyme HMG-CoA reductase. Several HPLC methods for its analysis have been developed but there is no report of its determination using capillary electrophoresis (CE). In this paper, we report the development of a simple CE method for lovastatin determination, which is selective with respect to its degradation products and useful for routine analyses. Since the molecule of lovastatin in its lactone form is uncharged and is only slightly soluble in water, base hydrolysis was used to open the lactone ring and transform the compound into a water-soluble acid form, which is negatively charged. Different solvents, different amounts of NaOH added, different hydrolysis times and different temperatures for sample preparation were tested. The CE and HPLC methods are compared in terms of susceptibility, precision, linearity and accuracy. HPLC method was found to be more susceptible and more precise.

Ključne riječi
lovastatin; lovastatin acid; analytical method; capillary electrophoresis, CE; high performance liquid chromatography, HPLC

Hrčak ID: 103177

URI
https://hrcak.srce.hr/103177

Posjeta: 1.828 *