hrcak mascot   Srce   HID

Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-64-2013-2313

CYP2E1 Testis Expression and Alcohol-Mediated Changes of Rat Spermatogenesis Indices and Type I Collagen

Ganna M. Shayakhmetova ; SI “Institute of Pharmacology & Toxicology” National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
Larysa B. Bondarenko ; SI “Institute of Pharmacology & Toxicology” National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
Valentina M. Kovalenko ; SI “Institute of Pharmacology & Toxicology” National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
Volodymyr V. Ruschak ; SI “Institute of Molecular Biology & Genetics” National Academy of Sciences of Ukraine2, Kyiv, Ukraine

Puni tekst: engleski, pdf (139 KB) str. 237-245 preuzimanja: 697* citiraj
APA 6th Edition
Shayakhmetova, G.M., Bondarenko, L.B., Kovalenko, V.M. i Ruschak, V.V. (2013). CYP2E1 Testis Expression and Alcohol-Mediated Changes of Rat Spermatogenesis Indices and Type I Collagen. Arhiv za higijenu rada i toksikologiju, 64 (2), 237-245. https://doi.org/10.2478/10004-1254-64-2013-2313
MLA 8th Edition
Shayakhmetova, Ganna M., et al. "CYP2E1 Testis Expression and Alcohol-Mediated Changes of Rat Spermatogenesis Indices and Type I Collagen." Arhiv za higijenu rada i toksikologiju, vol. 64, br. 2, 2013, str. 237-245. https://doi.org/10.2478/10004-1254-64-2013-2313. Citirano 20.09.2020.
Chicago 17th Edition
Shayakhmetova, Ganna M., Larysa B. Bondarenko, Valentina M. Kovalenko i Volodymyr V. Ruschak. "CYP2E1 Testis Expression and Alcohol-Mediated Changes of Rat Spermatogenesis Indices and Type I Collagen." Arhiv za higijenu rada i toksikologiju 64, br. 2 (2013): 237-245. https://doi.org/10.2478/10004-1254-64-2013-2313
Harvard
Shayakhmetova, G.M., et al. (2013). 'CYP2E1 Testis Expression and Alcohol-Mediated Changes of Rat Spermatogenesis Indices and Type I Collagen', Arhiv za higijenu rada i toksikologiju, 64(2), str. 237-245. https://doi.org/10.2478/10004-1254-64-2013-2313
Vancouver
Shayakhmetova GM, Bondarenko LB, Kovalenko VM, Ruschak VV. CYP2E1 Testis Expression and Alcohol-Mediated Changes of Rat Spermatogenesis Indices and Type I Collagen. Arh Hig Rada Toksikol. [Internet]. 2013 [pristupljeno 20.09.2020.];64(2):237-245. https://doi.org/10.2478/10004-1254-64-2013-2313
IEEE
G.M. Shayakhmetova, L.B. Bondarenko, V.M. Kovalenko i V.V. Ruschak, "CYP2E1 Testis Expression and Alcohol-Mediated Changes of Rat Spermatogenesis Indices and Type I Collagen", Arhiv za higijenu rada i toksikologiju, vol.64, br. 2, str. 237-245, 2013. [Online]. https://doi.org/10.2478/10004-1254-64-2013-2313

Sažetak
This study is a complex investigation of alcohol-mediated changes in CYP2E1 mRNA and protein expression in the testes, as well as spermatogenesis indices and type I collagen amino acid contents, in male rats. Wistar albino male rats were divided into two groups: I – control (intact animals), II – experimental (chronic alcoholism, exposure to a 15 % ethanol aqueous solution during 150 days). The destructive changes in the spermatogenic epithelium were accompanied by a decrease in sperm number and motility time. CYP2E1 mRNA and protein expression were elevated in the testes 3 and 1.4 times, respectively. Also, significantly lower contents of lysine, glutamic acid, serine, proline, alanine, valine, and phenylalanine residues accompanied by an increase of hydroxyproline, glycine, and threonine residue contents were detected in the skin type I collagen of the experimental group. Chronic ethanol consumption caused testicular failure along with an overexpression of CYP2E1 mRNA and protein in the testes as well as quantitative changes in type I collagen amino acid contents. The profound alcohol-mediated changes in collagen type I amino acid contents may have affected the spermatogenic epithelium state. The modulation of testicular cytochrome P450 2E1 mRNA and protein expression could change the functioning of this isozyme in target organs and take part in the mechanism of ethanol gonadotoxicity.

Ključne riječi
chronic alcoholism; ethanol; gonadotoxicity; motility

Hrčak ID: 103930

URI
https://hrcak.srce.hr/103930

[hrvatski]

Posjeta: 1.014 *