Izvorni znanstveni članak
Antibodies against GM1 Gangliosides Associated with Metastatic Melanoma
Corina Daniela Nicolae
; University of Medicine and Pharmacy, Bucharest, Romania
Ilinca Nicolae
; Victor Babes Hospital of Tropical and Infectious Diseases, Bucharest
Sažetak
The aim was to analyze humoral immune response against
GM1 ganglioside expressed on the surface of melanocytic cells, and the
possible correlation between the level of antibodies against GM1 IgG
and IgM class and melanoma progression. The study included 128 adult
patients with malignant melanoma, without paraneoplastic neurologic
disorders, 48 adults with dysplastic nevi and 48 healthy volunteers. The
presence of IgM and IgG antibodies against GM1 was demonstrated by
Immunodot method. Automatic evaluation of strips marked with GM1 antigen
was performed by EUROLineScan software. Lactate dehydrogenase
(LDH) activity was evaluated by spectrophotometry. Serum concentration
of gangliosides was determined using the method with resorcinol-HCl. IgG
antibodies against GM1 gangliosides were identified in six patients with
melanoma (4.68%) and in none of the subjects from other groups. AntiGM1
IgM class were observed in 20 (15.63%) melanoma patients, three (6.25%)
dysplastic nevi patients and one healthy volunteer. No statistically significant
difference was observed when serum profile of GM1 IgM antibodies in
patients with localized melanoma was compared with that of other study
subjects. The levels of IgM antibodies varied with clinical stage of tumor
and histopathologic features. Moreover, a statistically significant positive
correlation was found between IgM antibodies and LDH (r=0.87; p=0.01;
IC=95%). In conclusion, antibodies against GM1 ganglioside are frequent
in patients with melanoma. Dysplastic nevi and early melanoma cannot be
differentiated using the antiGM1 antibody profile. The synthesis of these
antibodies is characteristic for advanced stages of melanoma.
Ključne riječi
malignant melanoma; lactate-dehidrogenase; lipid associated sialic acid (LASA); GM1 antibodies
Hrčak ID:
106671
URI
Datum izdavanja:
23.8.2013.
Posjeta: 1.589 *