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https://doi.org/10.11613/BM.2013.036

Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder

Giuseppe Lippi   ORCID icon orcid.org/0000-0001-9523-9054 ; Clinical Chemistry Laboratory, Academic Hospital of Parma, Parma, Italy
Paola Avanzini ; Clinical Chemistry Laboratory, Academic Hospital of Parma, Parma, Italy
Rosalia Aloe ; Clinical Chemistry Laboratory, Academic Hospital of Parma, Parma, Italy
Gianfranco Cervellin ; Emergency Department, Academic Hospital of Parma, Parma, Italy

Puni tekst: engleski, pdf (187 KB) str. 303-307 preuzimanja: 392* citiraj
APA 6th Edition
Lippi, G., Avanzini, P., Aloe, R. i Cervellin, G. (2013). Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder. Biochemia Medica, 23 (3), 303-307. https://doi.org/10.11613/BM.2013.036
MLA 8th Edition
Lippi, Giuseppe, et al. "Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder." Biochemia Medica, vol. 23, br. 3, 2013, str. 303-307. https://doi.org/10.11613/BM.2013.036. Citirano 20.01.2021.
Chicago 17th Edition
Lippi, Giuseppe, Paola Avanzini, Rosalia Aloe i Gianfranco Cervellin. "Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder." Biochemia Medica 23, br. 3 (2013): 303-307. https://doi.org/10.11613/BM.2013.036
Harvard
Lippi, G., et al. (2013). 'Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder', Biochemia Medica, 23(3), str. 303-307. https://doi.org/10.11613/BM.2013.036
Vancouver
Lippi G, Avanzini P, Aloe R, Cervellin G. Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder. Biochemia Medica [Internet]. 2013 [pristupljeno 20.01.2021.];23(3):303-307. https://doi.org/10.11613/BM.2013.036
IEEE
G. Lippi, P. Avanzini, R. Aloe i G. Cervellin, "Reduction of gross hemolysis in catheter-drawn blood using Greiner Holdex® tube holder", Biochemia Medica, vol.23, br. 3, str. 303-307, 2013. [Online]. https://doi.org/10.11613/BM.2013.036

Sažetak
Introduction: Blood collection through intravenous lines frequently causes spurious hemolysis. Due to specific structure, the tube holder Holdex® (Greiner Bio-One GmbH, Kremsmuenster, Austria) is supposed to prevent erythrocyte injury in samples collected from catheters, so that we planned a specific study to support this hypothesis.
Materials and methods: Blood was collected from emergency department (ED) patients with 20-gauge catheter. In patients with odd order numbers, first and second tubes were collected with conventional holder (BD Vacutainer One Use Holder, Becton Dickinson, Milan, Italy) and the third with Holdex, whereas in even patients first and second tubes were drawn with Holdex and the third using BD Vacutainer One Use Holder. The first tube was discarded, whereas the second and third were centrifuged and serum was tested for potassium, lactate dehydrogenase (LD) and hemolysis index.
Results: The final study population consisted in 60 ED patients. Concentrations of potassium (4.25 vs. 4.16 mmol/L; P = 0.031), LD (498 vs. 459 U/L; P = 0.039) and cell-free hemoglobin (0.42 vs. 0.22 g/L; P = 0.042) were higher in samples collected with BD Vacutainer One Use Holder than with Holdex. The mean bias of cell-free hemoglobin was -0.4 g/L in samples collected with Holdex. Although the frequency of samples with cell-free hemoglobin > 0.5 g/L was identical (17/60 vs. 17/60; P = 1.00), the frequency of those with concentrations >3.0 g/L was higher using BD Vacutainer One Use Holder than Holdex (4/60 vs. 0/60; P = 0.042).
Conclusions: The use of Holdex for drawing blood from intravenous lines may be effective for reducing gross hemolysis.

Ključne riječi
preanalytical variability; hemolysis; sample quality; intravenous line

Hrčak ID: 109253

URI
https://hrcak.srce.hr/109253

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