Acta clinica Croatica, Vol. 52. No. 3., 2013.
Izvorni znanstveni članak
Effects of Dental Adhesives on Micronucleus Frequency in Peripheral Blood Lymphocytes In Vitro
Dunja Prica
Antonija Tadin
Danijela Marović
Marina Katunarić
Adriana Prica
Nada Galić
Sažetak
Dental adhesives come into direct contact with oral tissues. Due to this close and long-term contact, the materials should exhibit a high degree of biocompatibility. The aim of this study was to evaluate the genotoxic effect of dental adhesives on human lymphocytes in vitro. Polymerized dental adhesives (Excite, Adper Single Bond 2, Prompt L-pop and OptiBond Solo Plus) were eluted in dimethyl sulfoxide for 1 hour, 24 h and 120 h (5 days). Thereafter, lymphocyte cultures were treated with different concentrations of eluates (0.2 μg/mL, 0.5 μg/mL and 5 μg/mL) obtained from each of the tested materials. Genotoxicity was evaluated by micronucleus test. The χ2-test was used on statistical analysis (p<0.05). After elution period of 1 h, only the highest dose of all tested materials affected the measured cytogenetic parameters. After 24 h, genotoxicity was demonstrated only in cultures treated with eluates in concentrations of 0.5 μg/mL and 5 μg/mL. Based on the results, it is concluded that the use of dental adhesives causes genotoxic effects in human lymphocytes. Toxic effect of these dental adhesives increases with the tested material concentration and decreases with the length of elution period.
Ključne riječi
Dental adhesives; Mutagenicity tests; Lymphocytes; Micronucleus tests
Hrčak ID:
116705
URI
Datum izdavanja:
1.9.2013.
Posjeta: 1.303 *