Izvorni znanstveni članak
Genetic Diversity among Iranian Local and Commercial Apple Rootstocks by Using Simple Sequence Repeat Markers
Reza Darvishzadeh
; Department of Plant Breeding and Biotechnology, Urmia University, Urmia, Iran
Hamid Hatami Maleki
; Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
Javad Farrokhi
; Department of Horticulture, Urmia University, Urmia, Iran
Lotfali Naseri
; Department of Horticulture, Urmia University, Urmia, Iran
Sažetak
The objective of this research was to evaluate the genetic diversity among twelve apple rootstocks by using 14 simple sequence repeat primer pairs. Generally, 44 alleles were scored at SSR loci. The number of alleles (na) per locus ranged from two to five alleles with an average of 3.14. The maximum effective number of alleles (ne), expected heterozygosity (He), and observed heterozygosity (Hobs) were 2.3, 0.56 and 0.36, respectively. Results showed that the studied genotypes were not in Hardy-Weinberg equilibrium in most of studied loci. The polymorphism information content (PIC) varied from 0.5 to 0.86 which confirmed the effectiveness of SSR markers in determination of molecular polymorphism and characterization of the rootstocks genotypes. Cluster analysis using UPGMA algorithm on the basis of Jaccard’s similarity coefficient classified the studied genotypes into three distinct groups. In this study, three Iranian local rootstocks were located in separate groups.
Ključne riječi
apple rootstock; genetic diversity; simple sequence repeat marker; polymorphism information content; cluster analysis
Hrčak ID:
117942
URI
Datum izdavanja:
15.1.2014.
Posjeta: 786 *