hrcak mascot   Srce   HID

Pregledni rad

Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes

Ana Bielen ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Renata Teparić ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
Dušica Vujaklija ; Division of Molecular Biology, Institute Ruđer Bošković, Bijenička 54, HR-10000 Zagreb, Croatia
Vladimir Mrša ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (314 KB) str. 16-34 preuzimanja: 608* citiraj
APA 6th Edition
Bielen, A., Teparić, R., Vujaklija, D. i Mrša, V. (2014). Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes. Food Technology and Biotechnology, 52 (1), 16-34. Preuzeto s https://hrcak.srce.hr/118534
MLA 8th Edition
Bielen, Ana, et al. "Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes." Food Technology and Biotechnology, vol. 52, br. 1, 2014, str. 16-34. https://hrcak.srce.hr/118534. Citirano 16.01.2021.
Chicago 17th Edition
Bielen, Ana, Renata Teparić, Dušica Vujaklija i Vladimir Mrša. "Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes." Food Technology and Biotechnology 52, br. 1 (2014): 16-34. https://hrcak.srce.hr/118534
Harvard
Bielen, A., et al. (2014). 'Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes', Food Technology and Biotechnology, 52(1), str. 16-34. Preuzeto s: https://hrcak.srce.hr/118534 (Datum pristupa: 16.01.2021.)
Vancouver
Bielen A, Teparić R, Vujaklija D, Mrša V. Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes. Food Technology and Biotechnology [Internet]. 2014 [pristupljeno 16.01.2021.];52(1):16-34. Dostupno na: https://hrcak.srce.hr/118534
IEEE
A. Bielen, R. Teparić, D. Vujaklija i V. Mrša, "Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes", Food Technology and Biotechnology, vol.52, br. 1, str. 16-34, 2014. [Online]. Dostupno na: https://hrcak.srce.hr/118534. [Citirano: 16.01.2021.]
Puni tekst: hrvatski, pdf (275 KB) str. 16-34 preuzimanja: 135* citiraj
APA 6th Edition
Bielen, A., Teparić, R., Vujaklija, D. i Mrša, V. (2014). Mikrobni sustavi za izlaganje lipolitičkih enzima na površini stanice. Food Technology and Biotechnology, 52 (1), 16-34. Preuzeto s https://hrcak.srce.hr/118534
MLA 8th Edition
Bielen, Ana, et al. "Mikrobni sustavi za izlaganje lipolitičkih enzima na površini stanice." Food Technology and Biotechnology, vol. 52, br. 1, 2014, str. 16-34. https://hrcak.srce.hr/118534. Citirano 16.01.2021.
Chicago 17th Edition
Bielen, Ana, Renata Teparić, Dušica Vujaklija i Vladimir Mrša. "Mikrobni sustavi za izlaganje lipolitičkih enzima na površini stanice." Food Technology and Biotechnology 52, br. 1 (2014): 16-34. https://hrcak.srce.hr/118534
Harvard
Bielen, A., et al. (2014). 'Mikrobni sustavi za izlaganje lipolitičkih enzima na površini stanice', Food Technology and Biotechnology, 52(1), str. 16-34. Preuzeto s: https://hrcak.srce.hr/118534 (Datum pristupa: 16.01.2021.)
Vancouver
Bielen A, Teparić R, Vujaklija D, Mrša V. Mikrobni sustavi za izlaganje lipolitičkih enzima na površini stanice. Food Technology and Biotechnology [Internet]. 2014 [pristupljeno 16.01.2021.];52(1):16-34. Dostupno na: https://hrcak.srce.hr/118534
IEEE
A. Bielen, R. Teparić, D. Vujaklija i V. Mrša, "Mikrobni sustavi za izlaganje lipolitičkih enzima na površini stanice", Food Technology and Biotechnology, vol.52, br. 1, str. 16-34, 2014. [Online]. Dostupno na: https://hrcak.srce.hr/118534. [Citirano: 16.01.2021.]

Sažetak
Studies of microbial cell envelopes and particularly cell surface proteins and mechanisms of their localization brought about new biotechnological applications of the gained knowledge in surface display of homologous and heterologous proteins. By fusing surface
proteins or their anchoring domains with different proteins of interest, their so-called genetic immobilization is achieved. Hybrid proteins are engineered in a way that they are expressed in the host cells, secreted to the cell surface and incorporated into the wall/
envelope moiety. In this way, laborious and often detrimental procedure of chemical immobilization of the protein is avoided by letting the cells do the whole procedure. Both bacterial and yeast cells have been used for this purpose and a number of potential biotechnological applications of surface-displayed proteins have been reported. Among the most frequently used passenger proteins are lipolytic enzymes, due to their great technological significance and numerous important applications. In this review, our current knowledge on mechanisms and molecular systems for surface display of lipolytic enzymes on bacterial and yeast cell surfaces is summarized.

Ključne riječi
surface display; genetic immobilization; lipolytic enzymes; bacterial envelope; yeast cell wall

Hrčak ID: 118534

URI
https://hrcak.srce.hr/118534

[hrvatski]

Posjeta: 1.048 *