Veterinarski arhiv, Vol. 84 No. 3, 2014.
Izvorni znanstveni članak
The prevalence of methicillin resistance and Panton-Valentine leukocidin synthesis genes in Staphylococcus aureus isolates of bovine and human origin.
Marija J. Pajić
; Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, R. Serbia
Zoran B. Rašić
; Veterinary Institute “Jagodina”, Jagodina, R. Serbia
Branko M. Velebit
; Institute of Meat Hygiene and Technology, Belgrade, R. Serbia
Stanko F. Boboš
; Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, R. Serbia
Mira M. Mihajlović-Ukropina
; School of Medicine, University of Novi Sad, Novi Sad, R. Serbia
Miodrag Ž. Radinović
; Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, R. Serbia
Annamaria L. Galfi
; Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, R. Serbia
Jelena M. Petković
; Veterinary Institute “Jagodina”, Jagodina, R. Serbia
Snježana I. Trojačanec
; Department of Animal Physiology, Faculty of Agricultural Sciences and Food, University Ss. Cyril and Methodius, Skopje, R. Macedonia
Sažetak
Using the California Mastitis Test (CMT), on 46 highly productive dairy farms in Serbia, cows with milk secretion disorder were identified. Milk samples were taken from cows with positive CMT and from cows with clinical mastitis. Standard microbiological analysis of milk samples and API Staph confirmed the presence of 75 isolates of Staphylococcus aureus. Those 75 isolates, as well as 11 isolates of Staphylococcus aureus originating from humans were analyzed for the presence of genes encoding Panton-Valentine leukocidin (PVL) and PBP2A protein, responsible for methicillin resistance. The presence of gene encoding PVL was determined by PCR in 5 out of 75 (6.67%) and in 7 out of 11 (63.63%) bovine and human isolates of Staphylococcus aureus, respectively. The presence of the mecA gene was determined by PCR in 1 of 75 (1.33%) and in 2 of 11 (18.18%) bovine and human isolates of Staphylococcus aureus, respectively. The presence of the mecC gene was not determined in analyzed isolates. Further research is needed to investigate the genetic relationship between bovine and human Staphylococcus aureus isolates, to determine the exact impact of bovine Staphylococcus aureus strains from the cow udders on animal and public health.
Ključne riječi
cows; udder; milk; PantonValentine leukocidin; methicillin resistance
Hrčak ID:
121942
URI
Datum izdavanja:
28.5.2014.
Posjeta: 1.258 *