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Reproducibility of post-occlusion reactive hyperaemia assessed by laser Doppler flowmetry in young healthy women

LIDIJA RAŠIĆ orcid.org/0000-0003-0761-047X ; Department of Physiology and Immunology, Faculty of Medicine, Josip J. Strossmayer University of Osijek, Osijek, Croatia
ANA ČAVKA orcid.org/0000-0002-7446-0021 ; Department of Physiology and Immunology, Faculty of Medicine, Josip J. Strossmayer University of Osijek, Osijek, Croatia
FERENC BARI ; Department of Medical Physics and Informatics, Faculty of Medicine, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary
INES DRENJANČEVIĆ orcid.org/0000-0003-4964-7721 ; Department of Physiology and Immunology, Faculty of Medicine, Josip J. Strossmayer University of Osijek, J. Huttlera 4, HR-31 000, Osijek, Croatia

Sažetak

The aim of this study was to evaluate the day-to-day and inter-subject
reproducibility of post occlusive reactive hyperaemia (PORH) measured by
laser Doppler flowmetry (LDF) in young healthy female subjects. In addition,
we looked for most reproducible form of data presentation.
10 healthy female subjects were tested 4 times during 2 weeks. Blood flow
was measured during baseline, vascular occlusion (1, 2 and 3 min) and
PORH. Parameters studied were: increase in flow during vascular reperfusion
(R), difference between % of flow change during reperfusion and occlusion
(delta R-O) in relation to baseline, time-to-peak (TtP) and latency
time of the flow to go back to baseline levels (LT). Coefficient of variation
(CV; with 95% confidence interval) and intraclass correlation coefficient
(ICC; with 95% confidence interval) were calculated for each parameter.
CVs of ≤10%, 10–25% and ≥25% were considered good, moderate and
poor reproducibility. An ICC value of <0.40, 0.40–0.75 and >0.75 considered
as poor, fair-to-good and excellent reproducibility.
None of the parameters showed significant differences across the measurements.
Obtained CVs for all parameters have shown moderate reproducibility
(10-25%), except CV for TtP which was >25%. R-O was the most
reproducible parameter of reactive hyperemia, indicated by the lowest CV
and highest ICC (0.60), which is considered fair-to-good reproducibility.
The results of this study suggest that parameter R-O is fair-to-good reproducible
for LDF assessment of PORH in young healthy women and it can
be a useful and reliable noninvasive clinical measurement index of microvascular
function.

Ključne riječi

laser Doppler flowmetry; microcirculation; endothelium

Hrčak ID:

125475

URI

https://hrcak.srce.hr/125475

Datum izdavanja:

31.3.2014.

Posjeta: 1.303 *