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Influence of a medium pH and ionic strength on the adhesion of Streptococcus thermophilus microorganisms to human erythrocytes

MARIANA ANIKIEIEVA ; V.N. Karazin Kharkiv National University, 4 Svoboda Sq., Kharkiv, 61022, Ukraine
OLGA GORDIYENKO ; Institute for Problems of Cryobiology and Cryomedicine, NAS of Ukraine, 23 Pereyaslavskaya str., 61015, Kharkov, Ukraine

Puni tekst: engleski, pdf (265 KB) str. 89-93 preuzimanja: 767* citiraj
APA 6th Edition
ANIKIEIEVA, M. i GORDIYENKO, O. (2014). Influence of a medium pH and ionic strength on the adhesion of Streptococcus thermophilus microorganisms to human erythrocytes. Periodicum biologorum, 116 (1), 89-93. Preuzeto s https://hrcak.srce.hr/125478
MLA 8th Edition
ANIKIEIEVA, MARIANA i OLGA GORDIYENKO. "Influence of a medium pH and ionic strength on the adhesion of Streptococcus thermophilus microorganisms to human erythrocytes." Periodicum biologorum, vol. 116, br. 1, 2014, str. 89-93. https://hrcak.srce.hr/125478. Citirano 26.09.2021.
Chicago 17th Edition
ANIKIEIEVA, MARIANA i OLGA GORDIYENKO. "Influence of a medium pH and ionic strength on the adhesion of Streptococcus thermophilus microorganisms to human erythrocytes." Periodicum biologorum 116, br. 1 (2014): 89-93. https://hrcak.srce.hr/125478
Harvard
ANIKIEIEVA, M., i GORDIYENKO, O. (2014). 'Influence of a medium pH and ionic strength on the adhesion of Streptococcus thermophilus microorganisms to human erythrocytes', Periodicum biologorum, 116(1), str. 89-93. Preuzeto s: https://hrcak.srce.hr/125478 (Datum pristupa: 26.09.2021.)
Vancouver
ANIKIEIEVA M, GORDIYENKO O. Influence of a medium pH and ionic strength on the adhesion of Streptococcus thermophilus microorganisms to human erythrocytes. Periodicum biologorum [Internet]. 2014 [pristupljeno 26.09.2021.];116(1):89-93. Dostupno na: https://hrcak.srce.hr/125478
IEEE
M. ANIKIEIEVA i O. GORDIYENKO, "Influence of a medium pH and ionic strength on the adhesion of Streptococcus thermophilus microorganisms to human erythrocytes", Periodicum biologorum, vol.116, br. 1, str. 89-93, 2014. [Online]. Dostupno na: https://hrcak.srce.hr/125478. [Citirano: 26.09.2021.]

Sažetak
Background and Purpose: Diseases caused by bacterial pathogens are
the result of the host-pathogen interaction, therefore the contact of bacteria with erythrocytes is highly possible. The aim of this work was to study Streptococcus thermophilus adhesion on human erythrocytes depending on the various pH and ionic strength of the medium.

Materials and Methods: Streptococcus thermophilus bacterial cells
and human red blood cells have been selected. Erythrocytes were washed with buffer saline and precipitated by centrifugation. Dried bacterial cells were suspended, incubated, washed in buffer saline and precipitated. The precipitate of both types of cells was resuspended in 1:2 ratio in a buffer solution with different physicochemical characteristics. The number of bacteria adhered to each erythrocyte has been counted under light microscope in each field of view and adhesion coefficient has been calculated.

Results: Adhesion coefficient is strongly dependent on pH of the medium and reaches maximum at physiological pH for red blood cells. Data for the other examined pH values probabilistically differ at the same confidence level. Adhesion index was the highest in physiological saline (2.21 ± 0.87). Both increase or decrease in the ionic strength (0.2 M salt) resulted in the drop of adhesion rate, it becomes minimal (0.95 ± 0.63) in the medium with the lowest investigated ionic strength (0.25 M sucrose, 0.025 M salt).

Conclusions: The results of our experiments indicate that adhesion proceeds through initial nonspecific stage which plays an important role in the adhesion process and affects the possibility of the second irreversible stage of adhesion.

Hrčak ID: 125478

URI
https://hrcak.srce.hr/125478

Posjeta: 964 *