Izvorni znanstveni članak

Effect of low temperatures and ionizing irradiation upon physical-mechanical properties and connective-tissue structures of porcine fibrous pericardium and aortic valve leaflets

IRINA MIKHAILOVA ; Department of Experimental Cryomedicine, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, 23 Pereyaslavskaya str., Kharkov, Ukraine 61015
BORIS SANDOMIRSKY ; Department of Experimental Cryomedicine, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, 23 Pereyaslavskaya str., Kharkov, Ukraine 61015
ANNA GORLENKO ; Department of Experimental Cryomedicine, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Klochkovskaya Street 105a, #23, Kharkov, Ukraine 61015

Sažetak

Xenogeneic tissue devitalization is one of the creating methods of the tissuereplacing the biocompatible cell-free shells for the regenerative surgery. The work describes the possibility of applying the complex approach based on the continuous usage of cryo and radioactive (electron irradiation exposure) biological tissue damage effects. The pre-implant treatment provides sterilization and a possibility for the low temperature preservation of xenografts. After the transplantation such a cell-free xenoscaffold can be gradually replaced with the autogenic extracellular matrix from the recipient’s cells and forms a stable long-term structure of the biological prosthesis.


Fibrous pericardium (FP) and aortic valve leaflets (AVLs) were extracted
from the mature pig. The prepared tissues were rinsed with the sterile normal saline solution and frozen down to the liquid-nitrogen temperature. After one time placing on water-bath (37°C) they were exposed to electron irradiation within dosage range of 25-30 kGray and submerged into the liquid nitrogen vapors. After influence of low temperature and ionizing radiation, tissue morphological structure was assessed using the optical microscopy. Deformations, i.e. longitudinal and transverse monoaxial strength were performed to calculate the physical and mechanical properties of FP and AVLs.


Such a devitalization method of the FP and AVLs causes significant destructive changes in cell elements, however the spatial arrangement and structural integrity of the connective tissue fiber are preserved. Joint impact of low temperatures and ionizing radiation gives the synergetic effect, increasing the strength and elastic tissue properties. Freezing down to –196 °C and electron irradiation initiate formation of the intra- and intermolecular transverse cross-linking due to the binding activity of fibrous proteins. It leads to a more dense arrangement of the collagen fiber, adds strength to the implant and
provides the structural tissue stabilization. The authors believe that during the remodeling in the recipient organism, the biomaterial structure modified in such a manner can successfully prevent physiological tension.

Ključne riječi

fibrous pericardium; aortic valve leaflets; physical and mechanical properties; morphological structure; cell-free xenogeneic materials; freeze; ionizing irradiation; devitalization

Hrčak ID:

125481

URI

https://hrcak.srce.hr/125481

Datum izdavanja:

31.3.2014.

Posjeta: 955 *