Acta Pharmaceutica, Vol. 57 No. 3, 2007.
Kratko priopćenje
https://doi.org/10.2478/v10007-007-0030-8
Assay of flavonoid aglycones from species of the genus Sideritis (Lamiaceae) from Macedonia with HPLC UV DAD
BISERA JANESKA
MARINA STEFOVA
KALINA ALIPIEVA
Sažetak
Flavonoids obtained from Sideritis species (Lamiaceae), S. raeseri and S. scardica, grown in Macedonia were studied. Qualitative and quantitative analyses of the flavonoid aglycones were performed using high-performance liquid chromatography (HPLC) with a UV diode array detector. Extracts were prepared by acid hydrolysis in acetone, reextraction in ethyl acetate and evaporation to dryness; the residue dissolved in methanol was subjected to HPLC analysis.
Isoscutellarein, chryseriol and apigenin were identified in the extracts. Also, a 4’-methyl ether derivative of isoscutellarein was found, together with hypolaetin and its methyl ether derivative, which were identified according to previously isolated glycosides and literature data. Quantitation was performed using calibration with apigenin.
According to this screening analysis, the samples of the genus Sideritis from Macedonia are rich in polyhydroxy flavones and analogous with the previously studied Mediterranean Sideritis species from the Ibero-North African and Greek Sideritis species with respect to the presence of 8-OH flavones and their derivatives.
Ključne riječi
flavonoids; polyhydroxy flavones; Sideritis; HPLC-UV DAD
Hrčak ID:
12063
URI
Datum izdavanja:
1.9.2007.
Posjeta: 3.128 *