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Surfactant Supplementation to Enhance the Production of Vitamin K2 Metabolites in Shake Flask Cultures Using Escherichia sp. Mutant FM3-1709

Yan Liu ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Zhi-Ming Zheng ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Hong-Wei Qiu ; National Center for Biotechnology Development, 100036 Beijing, PR China
Gen-Hai Zhao ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Peng Wang ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Hui Liu ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Li Wang ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Zhe-Min Li ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
He-Fang Wu ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Hong-Xia Liu ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China
Mu Tan ; Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Anhui, 230031 Hefei, PR China


Puni tekst: hrvatski pdf 42 Kb

str. 269-275

preuzimanja: 228

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Puni tekst: engleski pdf 420 Kb

str. 269-275

preuzimanja: 458

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Sažetak

The effects of the addition of various surfactants on the cell growth and production of vitamin K2 metabolites, such as intracellular menaquinone-4 (MK-4), extracellular MK-4, intracellular MK-6 and extracellular MK-6, were studied in the submerged culture of Escherichia sp. All of the added surfactants caused the extension of the exponential phase. Betaine, polyoxyethylene oleyl ether and Tween-80 were favourable to the cell growth of Escherichia sp. mutant strain FM3-1709. The highest cell growth (Xmax), biomass production rate (Qx) and biomass yield (Yx/s) were (12.6±0.2) g/L, (0.21±0.01) g/(L·h) and (2.42± 0.02) g/g, respectively. The results show that the addition of all surfactants led to a lower production of intracellular MK-4, whereas the production of extracellular MK-4 increased remarkably. Among the five investigated surfactants, the addition of the nonionic surfactant polyoxyethylene oleyl ether (1.0 g/L) led to the highest production of extracellular MK-4 ((33.6±0.4) mg/L), MK-6 ((2.56±0.07) mg/L) and the highest yield of total MK-4
((47.6±0.4) mg/L) and MK-6 ((6.0±0.1) mg/L). The addition of polyoxyethylene oleyl ether proved to be more beneficial for the secretion of MK-4 than MK-6.

Ključne riječi

surfactant; vitamin K2; shake flask cultures

Hrčak ID:

126170

URI

https://hrcak.srce.hr/126170

Datum izdavanja:

15.9.2014.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.375 *