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https://doi.org/10.3325/cmj.2014.55.239

Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats

Judit Kalász ; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Enikő Pásztor Tóth ; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Beáta Bódi ; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Miklós Fagyas ; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Attila Tóth ; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Bhattoa Harjit Pal ; University of Debrecen, Faculty of Medicine, Department of Laboratory Medicine, Debrecen, Hungary
Sándor G. Vári ; Cedars-Sinai Medical Center, International Research and Innovation Management Program, Los Angeles, CA, USA
Marta Balog ; J. J. Strossmayer University of Osijek, School of Medicine Department of Medical Biology Osijek, Croatia
Marija Heffer ; J. J. Strossmayer University of Osijek, School of Medicine Department of Medical Biology Osijek, Croatia
Zoltán Papp ; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary
Attila Borbély ; University of Debrecen, Faculty of Medicine, Institute of Cardiology, Division of Clinical Physiology, Debrecen, Hungary

Puni tekst: engleski, pdf (744 KB) str. 239-249 preuzimanja: 366* citiraj
APA 6th Edition
Kalász, J., Pásztor Tóth, E., Bódi, B., Fagyas, M., Tóth, A., Harjit Pal, B., ... Borbély, A. (2014). Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats. Croatian medical journal, 55 (3), 239-249. https://doi.org/10.3325/cmj.2014.55.239
MLA 8th Edition
Kalász, Judit, et al. "Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats." Croatian medical journal, vol. 55, br. 3, 2014, str. 239-249. https://doi.org/10.3325/cmj.2014.55.239. Citirano 26.01.2020.
Chicago 17th Edition
Kalász, Judit, Enikő Pásztor Tóth, Beáta Bódi, Miklós Fagyas, Attila Tóth, Bhattoa Harjit Pal, Sándor G. Vári, et al. "Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats." Croatian medical journal 55, br. 3 (2014): 239-249. https://doi.org/10.3325/cmj.2014.55.239
Harvard
Kalász, J., et al. (2014). 'Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats', Croatian medical journal, 55(3), str. 239-249. https://doi.org/10.3325/cmj.2014.55.239
Vancouver
Kalász J, Pásztor Tóth E, Bódi B, Fagyas M, Tóth A, Harjit Pal B i sur. Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats. Croat Med J. [Internet]. 2014 [pristupljeno 26.01.2020.];55(3):239-249. https://doi.org/10.3325/cmj.2014.55.239
IEEE
J. Kalász, et al., "Single acute stress-induced progesterone and ovariectomy alter cardiomyocyte contractile function in female rats", Croatian medical journal, vol.55, br. 3, str. 239-249, 2014. [Online]. https://doi.org/10.3325/cmj.2014.55.239

Sažetak
Aim To assess how ovarian-derived sex hormones (in particular
progesterone) modify the effects of single acute
stress on the mechanical and biochemical properties of
left ventricular cardiomyocytes in the rat.
Methods Non-ovariectomized (control, n = 8) and ovariectomized
(OVX, n = 8) female rats were kept under normal
conditions or were exposed to stress (control-S, n = 8
and OVX-S, n = 8). Serum progesterone levels were measured
using a chemiluminescent immunoassay. Left ventricular
myocardial samples were used for isometric force
measurements and protein analysis. Ca2+-dependent active
force (Factive), Ca2+-independent passive force (Fpassive),
and Ca2+-sensitivity of force production were determined
in single, mechanically isolated, permeabilized cardiomyocytes.
Stress- and ovariectomy-induced alterations in myofilament
proteins (myosin-binding protein C [MyBP-C], troponin
I [TnI], and titin) were analyzed by sodium dodecyl
sulfate gel electrophoresis using protein and phosphoprotein
stainings. Results Serum progesterone levels were significantly increased
in stressed rats (control-S, 35.6 ± 4.8 ng/mL and
OVX-S, 21.9 ± 4.0 ng/mL) compared to control (10 ± 2.9
ng/mL) and OVX (2.8 ± 0.5 ng/mL) groups. Factive was higher
in the OVX groups (OVX, 25.9 ± 3.4 kN/m2 and OVX-S,
26.3 ± 3.0 kN/m2) than in control groups (control, 16.4 ± 1.2
kN/m2 and control-S, 14.4 ± 0.9 kN/m2). Regarding the
potential molecular mechanisms, Factive correlated with
MyBP-C phosphorylation, while myofilament Ca2+-sensitivity
inversely correlated with serum progesterone levels
when the mean values were plotted for all animal groups.
Fpassive was unaffected by any treatment.
Conclusion Stress increases ovary-independent synthesis
and release of progesterone, which may regulate Ca2+-sensitivity
of force production in left ventricular cardiomyocytes.
Stress and female hormones differently alter Ca2+-
dependent cardiomyocyte contractile force production,
which may have pathophysiological importance during
stress conditions affecting postmenopausal women.

Hrčak ID: 129839

URI
https://hrcak.srce.hr/129839

Posjeta: 497 *