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Conference paper

The High Resolution Structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase

Camillo Rosano ; Department of Physics-INFM and Advanced Biotechnology Center-IST, University of Genova, Largo Rosanna Benzi, 10; I-16132 Genova, Italy
Gaetano Izzo ; Department of Physics-INFM and Advanced Biotechnology Center-IST, University of Genova, Largo Rosanna Benzi, 10; I-16132 Genova, Italy
Laura Sturla ; Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV; 1. I-16132 Genova, Italy
Angela Bisso ; Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV; 1. I-16132 Genova, Italy
Michela Tonetti ; Department of Experimental Medicine, Section of Biochemistry, University of Genova, Viale Benedetto XV; 1. I-16132 Genova, Italy
Martino Bolognesi ; Department of Physics-INFM and Advanced Biotechnology Center-IST, University of Genova, Largo Rosanna Benzi, 10; I-16132 Genova, Italy

Fulltext: english, pdf (327 KB) pages 887-899 downloads: 270* cite
APA 6th Edition
Rosano, C., Izzo, G., Sturla, L., Bisso, A., Tonetti, M. & Bolognesi, M. (2000). The High Resolution Structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase. Croatica Chemica Acta, 73 (3), 887-899. Retrieved from https://hrcak.srce.hr/132032
MLA 8th Edition
Rosano, Camillo, et al. "The High Resolution Structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase." Croatica Chemica Acta, vol. 73, no. 3, 2000, pp. 887-899. https://hrcak.srce.hr/132032. Accessed 19 Oct. 2020.
Chicago 17th Edition
Rosano, Camillo, Gaetano Izzo, Laura Sturla, Angela Bisso, Michela Tonetti and Martino Bolognesi. "The High Resolution Structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase." Croatica Chemica Acta 73, no. 3 (2000): 887-899. https://hrcak.srce.hr/132032
Harvard
Rosano, C., et al. (2000). 'The High Resolution Structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase', Croatica Chemica Acta, 73(3), pp. 887-899. Available at: https://hrcak.srce.hr/132032 (Accessed 19 October 2020)
Vancouver
Rosano C, Izzo G, Sturla L, Bisso A, Tonetti M, Bolognesi M. The High Resolution Structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase. Croatica Chemica Acta [Internet]. 2000 [cited 2020 October 19];73(3):887-899. Available from: https://hrcak.srce.hr/132032
IEEE
C. Rosano, G. Izzo, L. Sturla, A. Bisso, M. Tonetti and M. Bolognesi, "The High Resolution Structure of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase", Croatica Chemica Acta, vol.73, no. 3, pp. 887-899, 2000. [Online]. Available: https://hrcak.srce.hr/132032. [Accessed: 19 October 2020]

Abstracts
GDP-4-keto-6-deoxy-D-mannose epimerase/reductase is a bifunctional enzyme involved in the biosynthesis of cell-surface structures, such as blood group antigens. Each subunit in the homodimeric enzyme consists of two domains. The N-terminal domain displays a Rossmann-fold topology and binds the NADP+ coenzyme. The C-terminal domain is held to bind the substrate. The holo-enzyme structure has been refined at 1.45 Å resolution, based on synchrotron data, to a final R-factor of 0.127 (Rfree = 0.167). The refined protein model highlights several residues involved in coenzyme recognition and binding and suggests that the enzyme belongs to the short-chain dehydrogenase protein homology family. Implications of the catalytic mechanism are discussed.

Keywords
fucose metabolism; enzyme structure; short-chain dehydrogenase; NADP+; epimerization; cell surface antigens; high resolution protein crystallography

Hrčak ID: 132032

URI
https://hrcak.srce.hr/132032

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