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Rapid Extraction of Human DNA Containing Humic Acid

Davorka Sutlović
Marija Definis Gojanović
Šimun Anđelinović

Puni tekst: engleski, pdf (316 KB) str. 117-120 preuzimanja: 2.979* citiraj
APA 6th Edition
Sutlović, D., Definis Gojanović, M. i Anđelinović, Š. (2007). Rapid Extraction of Human DNA Containing Humic Acid. Croatica Chemica Acta, 80 (1), 117-120. Preuzeto s https://hrcak.srce.hr/12832
MLA 8th Edition
Sutlović, Davorka, et al. "Rapid Extraction of Human DNA Containing Humic Acid." Croatica Chemica Acta, vol. 80, br. 1, 2007, str. 117-120. https://hrcak.srce.hr/12832. Citirano 29.09.2020.
Chicago 17th Edition
Sutlović, Davorka, Marija Definis Gojanović i Šimun Anđelinović. "Rapid Extraction of Human DNA Containing Humic Acid." Croatica Chemica Acta 80, br. 1 (2007): 117-120. https://hrcak.srce.hr/12832
Harvard
Sutlović, D., Definis Gojanović, M., i Anđelinović, Š. (2007). 'Rapid Extraction of Human DNA Containing Humic Acid', Croatica Chemica Acta, 80(1), str. 117-120. Preuzeto s: https://hrcak.srce.hr/12832 (Datum pristupa: 29.09.2020.)
Vancouver
Sutlović D, Definis Gojanović M, Anđelinović Š. Rapid Extraction of Human DNA Containing Humic Acid. Croatica Chemica Acta [Internet]. 2007 [pristupljeno 29.09.2020.];80(1):117-120. Dostupno na: https://hrcak.srce.hr/12832
IEEE
D. Sutlović, M. Definis Gojanović i Š. Anđelinović, "Rapid Extraction of Human DNA Containing Humic Acid", Croatica Chemica Acta, vol.80, br. 1, str. 117-120, 2007. [Online]. Dostupno na: https://hrcak.srce.hr/12832. [Citirano: 29.09.2020.]

Sažetak
The identification process of dead bodies or human remains is nowadays conducted in numerous
fields of forensic science, archeology and other judicial cases. A particular problem is the
isolation and DNA typing of human remains found in mass graves, due to the degradation process,
as well as post mortal DNA contamination with bacteria, fungi, humic acids, metals, etc.
In this study, the influence of humic acid (HA) on the DNA extraction and typing is investigated.
If present in the amplification reaction mix, humic acid inhibited DNA amplification,
but the addition of 50 mg PVPP (polyvinil-polypyrrolidone) to the reaction mixture before extraction
appeared to be optimal in overcoming this inhibition.

Ključne riječi
human DNA; extraction; humic acid; DNA inhibition

Hrčak ID: 12832

URI
https://hrcak.srce.hr/12832

Posjeta: 3.353 *