Infektološki glasnik, Vol. 33 No. 3, 2013.
Izvorni znanstveni članak
Enzyme immunoassay for separate detection of anti-HCV antibodies to individual HCV antigens as a confirmatory assay in diagnostics of HCV infection
Nataša Cetinić Balent
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Radojka Mikulić
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Oktavija Đaković Rode
orcid.org/0000-0001-8023-4314
; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Sažetak
Diagnostics of hepatitis C is based on the determination of anti-HCV antibodies by screening enzyme immunoassays (EIA). Each reactive anti-HCV screening result, according to recent guidelines, requires further determination of HCV RNA. In cases of negative HCV RNA findings, reactive anti-HCV results should be confirmed by immunoblotting (IB). Alternative confirmatory EIA with the ability to determine the antibodies against individual HCV antigens, which are comparable with the results of immunoblot tests, are commercially available.
The aim of this study was to present an EIA test, the EIA-Anti-HCV-Spectrum (DSI, Italy) and compare the test results with standard IB test.
We analyzed 50 randomly selected serum samples with reactive screening EIA (Monolisa HCV Ag-Ab ULTRA, Bio-Rad, France) results. In order to exclude false positives, serum samples were further tested with confirmatory IB test (Deciscan HCV PLUS, Bio-Rad, France). A total of 50 selected samples were tested with EIA-Anti-HCV-Spectrum for separate detection of anti-HCV antibodies against core, NS3, NS4 and NS5 antigens.
Out of the 50 primary reactive samples, anti-HCV antibodies were positive in both confirmatory tests in 40, negative in 1 and borderline in 3 samples. Discordant results of confirmatory tests were recorded in 6 samples: all borderline in IB test and negative in the alternative EIA-Anti-HCV-Spectrum test.
We present an EIA confirmatory test for anti-HCV antibodies which showed a good correlation with the standard immunoblot test, although it is ecessary to perform the evaluation on a large number of samples for safer conclusions.
Ključne riječi
Hepatitis C virus (HCV); HCV diagnostic; anti-HCV; enzyme immunoassay (EIA); immunoblotting (IB); HCV RNA
Hrčak ID:
133432
URI
Datum izdavanja:
30.9.2013.
Posjeta: 3.618 *