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Comparison of sensitivity of nested PCR and quantitative PCR in Bcr-Abl p210 transcript detection in chronic myelogenous leukemia

Maruška Marušić Vrsalović
Rajko Kušec
Vlatko Pejša
Željko Romić

Puni tekst: engleski, pdf (299 KB) str. 109-114 preuzimanja: 379* citiraj
APA 6th Edition
Marušić Vrsalović, M., Kušec, R., Pejša, V. i Romić, Ž. (2007). Comparison of sensitivity of nested PCR and quantitative PCR in Bcr-Abl p210 transcript detection in chronic myelogenous leukemia. Biochemia Medica, 17 (1), 109-114. Preuzeto s https://hrcak.srce.hr/12919
MLA 8th Edition
Marušić Vrsalović, Maruška, et al. "Comparison of sensitivity of nested PCR and quantitative PCR in Bcr-Abl p210 transcript detection in chronic myelogenous leukemia." Biochemia Medica, vol. 17, br. 1, 2007, str. 109-114. https://hrcak.srce.hr/12919. Citirano 27.01.2021.
Chicago 17th Edition
Marušić Vrsalović, Maruška, Rajko Kušec, Vlatko Pejša i Željko Romić. "Comparison of sensitivity of nested PCR and quantitative PCR in Bcr-Abl p210 transcript detection in chronic myelogenous leukemia." Biochemia Medica 17, br. 1 (2007): 109-114. https://hrcak.srce.hr/12919
Harvard
Marušić Vrsalović, M., et al. (2007). 'Comparison of sensitivity of nested PCR and quantitative PCR in Bcr-Abl p210 transcript detection in chronic myelogenous leukemia', Biochemia Medica, 17(1), str. 109-114. Preuzeto s: https://hrcak.srce.hr/12919 (Datum pristupa: 27.01.2021.)
Vancouver
Marušić Vrsalović M, Kušec R, Pejša V, Romić Ž. Comparison of sensitivity of nested PCR and quantitative PCR in Bcr-Abl p210 transcript detection in chronic myelogenous leukemia. Biochemia Medica [Internet]. 2007 [pristupljeno 27.01.2021.];17(1):109-114. Dostupno na: https://hrcak.srce.hr/12919
IEEE
M. Marušić Vrsalović, R. Kušec, V. Pejša i Ž. Romić, "Comparison of sensitivity of nested PCR and quantitative PCR in Bcr-Abl p210 transcript detection in chronic myelogenous leukemia", Biochemia Medica, vol.17, br. 1, str. 109-114, 2007. [Online]. Dostupno na: https://hrcak.srce.hr/12919. [Citirano: 27.01.2021.]
Puni tekst: hrvatski, pdf (299 KB) str. 109-114 preuzimanja: 430* citiraj
APA 6th Edition
Marušić Vrsalović, M., Kušec, R., Pejša, V. i Romić, Ž. (2007). Usporedba osjetljivosti ugnježđene PCR i kvantitativne PCR u određivanju Bcr-Abl p210 prijepisa kronične mijeloične leukemije. Biochemia Medica, 17 (1), 109-114. Preuzeto s https://hrcak.srce.hr/12919
MLA 8th Edition
Marušić Vrsalović, Maruška, et al. "Usporedba osjetljivosti ugnježđene PCR i kvantitativne PCR u određivanju Bcr-Abl p210 prijepisa kronične mijeloične leukemije." Biochemia Medica, vol. 17, br. 1, 2007, str. 109-114. https://hrcak.srce.hr/12919. Citirano 27.01.2021.
Chicago 17th Edition
Marušić Vrsalović, Maruška, Rajko Kušec, Vlatko Pejša i Željko Romić. "Usporedba osjetljivosti ugnježđene PCR i kvantitativne PCR u određivanju Bcr-Abl p210 prijepisa kronične mijeloične leukemije." Biochemia Medica 17, br. 1 (2007): 109-114. https://hrcak.srce.hr/12919
Harvard
Marušić Vrsalović, M., et al. (2007). 'Usporedba osjetljivosti ugnježđene PCR i kvantitativne PCR u određivanju Bcr-Abl p210 prijepisa kronične mijeloične leukemije', Biochemia Medica, 17(1), str. 109-114. Preuzeto s: https://hrcak.srce.hr/12919 (Datum pristupa: 27.01.2021.)
Vancouver
Marušić Vrsalović M, Kušec R, Pejša V, Romić Ž. Usporedba osjetljivosti ugnježđene PCR i kvantitativne PCR u određivanju Bcr-Abl p210 prijepisa kronične mijeloične leukemije. Biochemia Medica [Internet]. 2007 [pristupljeno 27.01.2021.];17(1):109-114. Dostupno na: https://hrcak.srce.hr/12919
IEEE
M. Marušić Vrsalović, R. Kušec, V. Pejša i Ž. Romić, "Usporedba osjetljivosti ugnježđene PCR i kvantitativne PCR u određivanju Bcr-Abl p210 prijepisa kronične mijeloične leukemije", Biochemia Medica, vol.17, br. 1, str. 109-114, 2007. [Online]. Dostupno na: https://hrcak.srce.hr/12919. [Citirano: 27.01.2021.]

Sažetak
Background: For minimal residual disease detection in chronic myelogenous leukemia (CML) patients who have achieved complete clinical remission and complete cytogenetic response, nested PCR (nPCR) and quantitative realtime PCR (qPCR) can be used. Achieving of molecular remission is the goal of therapy, so it is of critical importance and clinical utility to obtain high sensitivity of molecular testing. We compared the level of sensitivity of nPCR and qPCR in the detection of BCR-ABL p210 transcripts in a Bcr/Abl-positive cell dilution model.
Materials and Methods: For determination of sensitivity level, serial dilutions of K562 cell line (Bcr-Abl-positive) in NB4cell line (Bcr-Abl-negative) were made (range of dilution of positive cells: 10-3-10-7). Isolated RNA samples were transcribed into cDNA and tested for p210 transcript by nPCR and qPCR. Bone marrow and peripheral blood samples of two CML patients on imatinib mesylate therapy were also tested by both methods.
Results: In the cell dilution test, nPCR showed sensitivity for detecting Bcr-Abl positive cell of 10-5, and qPCR showed a sensitivity of 10-6. Both methods detected p210 transcripts in bone marrow samples of CML patients. However, qPCR also detected transcripts in peripheral blood samples, with a lower transcript level in comparison to bone marrow samples.
Conclusion: Although frequently quoted as nPCR being by approximately 1 log more sensitive than qPCR, in our marker-positive cell line K562 dilution experiment we documented higher sensitivity of the standardized Europe Against Cancer Program developed qPCR method.

Ključne riječi
nPCR; qPCR; sensitivity; p210 detection

Hrčak ID: 12919

URI
https://hrcak.srce.hr/12919

[hrvatski]

Posjeta: 1.665 *