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Ecto-ADPase Activity in the Rat Renal Brush-Border Membranes

Tihana Žanić-Grubišić ; Department of Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, P.O. Box 156, HR-10 000 Zagreb, Croatia
Lorena Griparić ; Department of Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, P.O. Box 156, HR-10 000 Zagreb, Croatia
Renata Zrinski ; Department of Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, P.O. Box 156, HR-10 000 Zagreb, Croatia
Mima Floegel ; Department of Medical Biochemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovačića 1, P.O. Box 156, HR-10 000 Zagreb, Croatia

Puni tekst: engleski, pdf (29 MB) str. 491-510 preuzimanja: 112* citiraj
APA 6th Edition
Žanić-Grubišić, T., Griparić, L., Zrinski, R. i Floegel, M. (1995). Ecto-ADPase Activity in the Rat Renal Brush-Border Membranes. Croatica Chemica Acta, 68 (3), 491-510. Preuzeto s https://hrcak.srce.hr/136710
MLA 8th Edition
Žanić-Grubišić, Tihana, et al. "Ecto-ADPase Activity in the Rat Renal Brush-Border Membranes." Croatica Chemica Acta, vol. 68, br. 3, 1995, str. 491-510. https://hrcak.srce.hr/136710. Citirano 03.03.2021.
Chicago 17th Edition
Žanić-Grubišić, Tihana, Lorena Griparić, Renata Zrinski i Mima Floegel. "Ecto-ADPase Activity in the Rat Renal Brush-Border Membranes." Croatica Chemica Acta 68, br. 3 (1995): 491-510. https://hrcak.srce.hr/136710
Harvard
Žanić-Grubišić, T., et al. (1995). 'Ecto-ADPase Activity in the Rat Renal Brush-Border Membranes', Croatica Chemica Acta, 68(3), str. 491-510. Preuzeto s: https://hrcak.srce.hr/136710 (Datum pristupa: 03.03.2021.)
Vancouver
Žanić-Grubišić T, Griparić L, Zrinski R, Floegel M. Ecto-ADPase Activity in the Rat Renal Brush-Border Membranes. Croatica Chemica Acta [Internet]. 1995 [pristupljeno 03.03.2021.];68(3):491-510. Dostupno na: https://hrcak.srce.hr/136710
IEEE
T. Žanić-Grubišić, L. Griparić, R. Zrinski i M. Floegel, "Ecto-ADPase Activity in the Rat Renal Brush-Border Membranes", Croatica Chemica Acta, vol.68, br. 3, str. 491-510, 1995. [Online]. Dostupno na: https://hrcak.srce.hr/136710. [Citirano: 03.03.2021.]

Sažetak
Brush-border membrane vesicles purified from rat kidney cortex exhibit an ectoenzyme activity responsible for the hydrolysis of both ATP and ADP, as well as of other nucleoside tri- and diphosphates. In the presence of Ca2+ ions, ADP hydrolysis follows the simple Michaelis-Menten kinetics assuming a single catalytic site.
The real substrate for ADPase is a divalent cation conjugated ADP.
The pH optimum for the hydrolysis is between 7.2 and 8.6.
ADP and ATP hydrolysis show similar heat denaturation curves, and are both resistant to limited proteolysis and to inhibitors of other known ATPases. The enzyme activity is inhibited by: diethyl py- rocarbonate, dithiothreitol, high concentrations of both IV-ethylmale- imide and azide. The diethyl pyrocarbonate inhibition could be reversed by hydroxylamine, indicating the involvement of histidine and/or tyrosine residues in the reaction. It is proposed that both ADPase and ATPase activities reside within the same enzyme protein.

Hrčak ID: 136710

URI
https://hrcak.srce.hr/136710

Posjeta: 220 *