Probing of the Surface of High-Density Lipoproteins with Mn(II) Ions and EPR Spectroscopy
Jasminka Brnjas-Kraljević
; School of Medicine, University of Zagreb, Zagreb, Croatia
Greta Pifat
; Rudjer Bošković Institute
Janko Herak
; Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia
APA 6th Edition Brnjas-Kraljević, J., Pifat, G. i Herak, J. (1993). Probing of the Surface of High-Density Lipoproteins with Mn(II) Ions and EPR Spectroscopy. Croatica Chemica Acta, 66 (3-4), 547-554. Preuzeto s https://hrcak.srce.hr/137056
MLA 8th Edition Brnjas-Kraljević, Jasminka, et al. "Probing of the Surface of High-Density Lipoproteins with Mn(II) Ions and EPR Spectroscopy." Croatica Chemica Acta, vol. 66, br. 3-4, 1993, str. 547-554. https://hrcak.srce.hr/137056. Citirano 27.02.2021.
Chicago 17th Edition Brnjas-Kraljević, Jasminka, Greta Pifat i Janko Herak. "Probing of the Surface of High-Density Lipoproteins with Mn(II) Ions and EPR Spectroscopy." Croatica Chemica Acta 66, br. 3-4 (1993): 547-554. https://hrcak.srce.hr/137056
Harvard Brnjas-Kraljević, J., Pifat, G., i Herak, J. (1993). 'Probing of the Surface of High-Density Lipoproteins with Mn(II) Ions and EPR Spectroscopy', Croatica Chemica Acta, 66(3-4), str. 547-554. Preuzeto s: https://hrcak.srce.hr/137056 (Datum pristupa: 27.02.2021.)
Vancouver Brnjas-Kraljević J, Pifat G, Herak J. Probing of the Surface of High-Density Lipoproteins with Mn(II) Ions and EPR Spectroscopy. Croatica Chemica Acta [Internet]. 1993 [pristupljeno 27.02.2021.];66(3-4):547-554. Dostupno na: https://hrcak.srce.hr/137056
IEEE J. Brnjas-Kraljević, G. Pifat i J. Herak, "Probing of the Surface of High-Density Lipoproteins with Mn(II) Ions and EPR Spectroscopy", Croatica Chemica Acta, vol.66, br. 3-4, str. 547-554, 1993. [Online]. Dostupno na: https://hrcak.srce.hr/137056. [Citirano: 27.02.2021.]
Sažetak Negatively charged sites on the surface of two human high-density lipoproteins, HDL2 and HDL3, have been studied using EPR spectroscopy. Like for LDL, there are two types of binding sites for the Mn(II) ions on each HDL class, characterized by essentially the same binding constants as those for LDL. The small number of »strong« sites (n = 2), attributed to the negatively charged amino-acid residues, indicates that only a small fraction of these residues is available for cation binding. The binding constants for »weak« sites and the number of these sites in LDL and in the two HDL subfractions studied are consistent with the idea that these sites relate to the phospholipid domains on the lipoprotein surface.