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https://doi.org/10.5562/cca2514

Characterization of Summer Savory (Satureja hortensis L.) Honey by Physico-Chemical Parameters and Chromatographic / Spectroscopic Techniques (GC-FID/MS, HPLC-DAD, UV/VIS and FTIR-ATR)

Igor Jerković ; Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, HR-21000 Split, Croatia
Carlo Ignazio Giovanni Tuberoso ; Department of Life and Environmental Sciences, University of Cagliari, via Ospedale 72, IT-09124 Cagliari, Italy
Goran Baranović ; Ruđer Bošković Institute, Division of Organic Chemistry and Biochemistry, HR-10000 Zagreb, Croatia
Zvonimir Marijanović ; Department of Food Technology, Marko Marulić Polytechnic in Knin, Petra Krešimira IV 30, HR-22300 Knin, Croatia
Marina Kranjac ; Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, N. Tesle 10/V, HR-21000 Split, Croatia
Lidija Svečnjak ; Department of Fisheries, Apiculture and Special Zoology, Faculty of Agriculture, University of Zagreb, HR-10000 Zagreb, Croatia
Piotr Marek Kuś ; Department of Pharmacognosy, Wrocław Medical University, ul. Borowska 211a, PL-50-556 Wrocław, Poland

Sažetak

Satureja hortensis L. unifloral honey was characterized by pollen analysis, electrical conductivity, pH and extensively by chromatographic and spectroscopic techniques. UV / VIS spectro-scopy measurements revealed total phenol content of 682.1 mg GAE / kg by Folin-Ciocalteu assay, antiox-idant capacity by DPPH assay of 1.7 mmol TEAC / kg and by FRAP assay of 4.3 mmol Fe2+ / kg as well as CIE L*a*b*Cab*h°ab chromaticity coordinates. GC-MS after headspace solid-phase microextraction (HS-SPME) revealed hotrienol (22.8 %) along with other linalool derivatives, benzaldehyde (6.1 %), phenylacetaldehyde (4.9 %) and few norisoprenoids (safranal (7.6 %) as the major). Ultrasonic solvent ex-traction (USE) followed by GC-MS allowed identification of methyl syringate (54.7 %) as predominant compound along with other benzene derivatives. HPLC-DAD analysis determined tyrosine (382.0 mg kg−1), phenylalanine (140.4 mg kg−1) and methyl syringate (39.32 mg kg−1). Methyl syringate and hotrienol can be considered non-specific chemical markers of S. hortensis honey. FTIR-ATR spectral characteristics of S. hortensis honey in fingerprinting region were not significantly different from other honey types, but the integrated intensity of the region was smaller than in other unifloral honeys.

Ključne riječi

Satureja hortensis L. honey; GC-MS; HPLC-DAD; UV / VIS; FTIR-ATR; methyl syringate; hotrienol

Hrčak ID:

139125

URI

https://hrcak.srce.hr/139125

Datum izdavanja:

27.3.2015.

Posjeta: 2.896 *