Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2014.2251
Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids
S. Follonier
; Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais (HES-SO Valais-Wallis), Route du Rawyl 64, 1950 Sion, Switzerland
R. Riesen
; Changins, Route de Duillier 50, Case postale 1148, 1260 Nyon 1, Switzerland
M. Zinn
orcid.org/0000-0003-3565-3872
; Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais (HES-SO Valais-Wallis), Route du Rawyl 64, 1950 Sion, Switzerland
Sažetak
Bioprocess optimization is a prime target to decrease the cost of functionalized medium-chain-length poly(3-hydroxyalkanoate) (mcl-PHA). We have already demonstrated at the laboratory scale that the pomace of white wine grapes is a promising growth substrate for the biosynthesis of mcl-PHA that is both cheap and does not compete with food and land use. Here, we report the scale-up of a 2-step, 100 L bioprocess with Pseudomonas putida KT2440 involving: (1) a batch growth phase on extract of Gewürztraminer grape pomace, and (2) a fed-batch polymer accumulation phase with a linear feed of 50 mol % octanoic acid and 50 mol % 10-undecenoic acid. With this approach, we achieved a cell dry weight of 14.2 ± 0.3 g L–1 containing 41.1 ± 1.3 wt % of poly(3-hydroxyoctanoate-co-3-hydroxy-10-undecenoate) with 53 mol % and 47 mol % of saturated and unsaturated monomers, respectively. The molecular weight (Mw) was 139 000 Da (PDI = 1.97) and DSC analysis showed a Tg of –45.5 °C but no Tm thus indicating a completely amorphous polymer.
Ključne riječi
polyhydroxyalkanoate; mcl-PHA; scale-up; pomace; functionalized PHA
Hrčak ID:
141906
URI
Datum izdavanja:
18.7.2015.
Posjeta: 2.331 *