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Original scientific paper
https://doi.org/10.15255/CABEQ.2014.2251

Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids

S. Follonier ; Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais (HES-SO Valais-Wallis), Route du Rawyl 64, 1950 Sion, Switzerland
R. Riesen ; Changins, Route de Duillier 50, Case postale 1148, 1260 Nyon 1, Switzerland
M. Zinn   ORCID icon orcid.org/0000-0003-3565-3872 ; Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland Valais (HES-SO Valais-Wallis), Route du Rawyl 64, 1950 Sion, Switzerland

Fulltext: english, pdf (1 MB) pages 113-121 downloads: 401* cite
APA 6th Edition
Follonier, S., Riesen, R. & Zinn, M. (2015). Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids. Chemical and Biochemical Engineering Quarterly, 29 (2), 113-121. https://doi.org/10.15255/CABEQ.2014.2251
MLA 8th Edition
Follonier, S., et al. "Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids." Chemical and Biochemical Engineering Quarterly, vol. 29, no. 2, 2015, pp. 113-121. https://doi.org/10.15255/CABEQ.2014.2251. Accessed 20 Jun. 2019.
Chicago 17th Edition
Follonier, S., R. Riesen and M. Zinn. "Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids." Chemical and Biochemical Engineering Quarterly 29, no. 2 (2015): 113-121. https://doi.org/10.15255/CABEQ.2014.2251
Harvard
Follonier, S., Riesen, R., and Zinn, M. (2015). 'Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids', Chemical and Biochemical Engineering Quarterly, 29(2), pp. 113-121. doi: https://doi.org/10.15255/CABEQ.2014.2251
Vancouver
Follonier S, Riesen R, Zinn M. Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids. Chemical and Biochemical Engineering Quarterly [Internet]. 2015 [cited 2019 June 20];29(2):113-121. doi: https://doi.org/10.15255/CABEQ.2014.2251
IEEE
S. Follonier, R. Riesen and M. Zinn, "Pilot-scale Production of Functionalized mcl-PHA from Grape Pomace Supplemented with Fatty Acids", Chemical and Biochemical Engineering Quarterly, vol.29, no. 2, pp. 113-121, 2015. [Online]. doi: https://doi.org/10.15255/CABEQ.2014.2251

Abstracts
Bioprocess optimization is a prime target to decrease the cost of functionalized medium-chain-length poly(3-hydroxyalkanoate) (mcl-PHA). We have already demonstrated at the laboratory scale that the pomace of white wine grapes is a promising growth substrate for the biosynthesis of mcl-PHA that is both cheap and does not compete with food and land use. Here, we report the scale-up of a 2-step, 100 L bioprocess with Pseudomonas putida KT2440 involving: (1) a batch growth phase on extract of Gewürztraminer grape pomace, and (2) a fed-batch polymer accumulation phase with a linear feed of 50 mol % octanoic acid and 50 mol % 10-undecenoic acid. With this approach, we achieved a cell dry weight of 14.2 ± 0.3 g L–1 containing 41.1 ± 1.3 wt % of poly(3-hydroxyoctanoate-co-3-hydroxy-10-undecenoate) with 53 mol % and 47 mol % of saturated and unsaturated monomers, respectively. The molecular weight (Mw) was 139 000 Da (PDI = 1.97) and DSC analysis showed a Tg of –45.5 °C but no Tm thus indicating a completely amorphous polymer.

Keywords
polyhydroxyalkanoate; mcl-PHA; scale-up; pomace; functionalized PHA

Hrčak ID: 141906

URI
https://hrcak.srce.hr/141906

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