Izvorni znanstveni članak
Enhanced Detection of Human Plasma Proteins on Nanostructured Silver Surfaces
Zuzana Orságová Králová
; Department of Physical Chemistry, Faculty of Science, University of P.J. Šafárik in Košice, Košice, Slovakia
Andrej Orinák
; Department of Physical Chemistry, Faculty of Science, University of P.J. Šafárik in Košice, Košice, Slovakia
Renáta Orináková
; Department of Physical Chemistry, Faculty of Science, University of P.J. Šafárik in Košice, Košice, Slovakia
Lenka Škántarová
; Department of Analytical Chemistry, Comenius University, Bratislava, Slovakia
Jozef Radonák
; Faculty of Human Medicine, University of P.J. Šafárik in Košice, Košice, Slovakia
Sažetak
In chemical and medical research, recent methods combine the tools of nanotechnology, chemistry and biology in a way that introduces the most modern processes to current medical practice. The main blood plasma proteins – albumin and globulin and their amino acid sequences, are carriers of important information about human health. In this paper we employed silver nanostructured surfaces prepared by electrodeposition. Consequently, electrochemical deposition is introduced as a convenient, fast and cost‐effective method for the preparation of metallic nanostructures with required morphology. Silver nanostructured surfaces were applied as the templates for Surface Enhanced Raman Spectroscopy (SERS) of albumin and globulin in the role of model analytes. We also studied the effect of a working electrode polishing process on electrodeposition and identification of proteins. The aqueous solutions of albumin and globulin were applied onto these Ag nanostructured substrates separately. An analytical signal enhancement factor of 3.6×102 was achieved for a band with a Raman shift of 2104cm‐1 for globulin deposited onto silver nanostructured film on unpolished stainless steel substrate. The detection limit was 400µg/mL. Plasma or serum could present a preferable material for non‐invasive cancer disease diagnosis using the SERS method.
Ključne riječi
electrochemical deposition; nanostructured surfaces; albumin; globulin, surface enhanced Raman spectroscopy (SERS)
Hrčak ID:
142837
URI
Datum izdavanja:
1.1.2013.
Posjeta: 901 *