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https://doi.org/10.5772/53926

Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins

J. J. Beato-López ; Departamento de Fisica de la Materia Condensada, Universidad de Cádiz, Cádiz, Spain
C. Fernández-Ponce ; Departamento de Biomedicina, Biotecnologia y Salud Publica, Universidad de Cádiz, Cádiz, Spain
E. Blanco ; Departamento de Fisica de la Materia Condensada, Universidad de Cádiz, Cádiz, Spain
C. Barrera-Solano ; Departamento de Fisica de la Materia Condensada, Universidad de Cádiz, Cádiz, Spain
M. Ramirez-del-Solar ; Departamento de Fisica de la Materia Condensada, Universidad de Cádiz, Cádiz, Spain
M. Dominguez ; Departamento de Fisica de la Materia Condensada, Universidad de Cádiz, Cádiz, Spain
F. García-Cozar ; Departamento de Biomedicina, Biotecnologia y Salud Publica, Universidad de Cádiz, Cádiz, Spain
R. Litrán ; Departamento de Fisica de la Materia Condensada, Universidad de Cádiz, Cádiz, Spain

Puni tekst: engleski, pdf (2 MB) str. 2-10 preuzimanja: 1.784* citiraj
APA 6th Edition
Beato-López, J.J., Fernández-Ponce, C., Blanco, E., Barrera-Solano, C., Ramirez-del-Solar, M., Dominguez, M., ... Litrán, R. (2012). Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins. Nanomaterials and Nanotechnology, 2 (Godište 2012), 2-10. https://doi.org/10.5772/53926
MLA 8th Edition
Beato-López, J. J., et al. "Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins." Nanomaterials and Nanotechnology, vol. 2, br. Godište 2012, 2012, str. 2-10. https://doi.org/10.5772/53926. Citirano 18.09.2021.
Chicago 17th Edition
Beato-López, J. J., C. Fernández-Ponce, E. Blanco, C. Barrera-Solano, M. Ramirez-del-Solar, M. Dominguez, F. García-Cozar i R. Litrán. "Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins." Nanomaterials and Nanotechnology 2, br. Godište 2012 (2012): 2-10. https://doi.org/10.5772/53926
Harvard
Beato-López, J.J., et al. (2012). 'Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins', Nanomaterials and Nanotechnology, 2(Godište 2012), str. 2-10. https://doi.org/10.5772/53926
Vancouver
Beato-López JJ, Fernández-Ponce C, Blanco E, Barrera-Solano C, Ramirez-del-Solar M, Dominguez M i sur. Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins. Nanomaterials and Nanotechnology [Internet]. 2012 [pristupljeno 18.09.2021.];2(Godište 2012):2-10. https://doi.org/10.5772/53926
IEEE
J.J. Beato-López, et al., "Preparation and Characterization of Fluorescent CdS Quantum Dots used for the Direct Detection of GST Fusion Proteins", Nanomaterials and Nanotechnology, vol.2, br. Godište 2012, str. 2-10, 2012. [Online]. https://doi.org/10.5772/53926

Sažetak
Advances in the life sciences are now closely linked to the availability of new experimental tools that allow for the manipulation of biomolecules and the study of biological processes at the molecular level. In this context, we have optimized a synthesis process to obtain glutathione‐capped fluorescent CdS nanoparticles to specifically detect Glutathione S‐Transferase (GST) ‐tagged proteins. Using our method, based on five different heating steps, brightly fluorescent and biocompatible CdS quantum dots of different sizes can be obtained. QD optical behaviour has been evaluated studying both absorbance and fluorescence. For all the samples, the excitonic absorption onset clearly shows a blue shift at 512nm in comparison with that of bulk CdS, due to the quantum confinement effect. At increased average sizes of the nanocrystal, the emission fluorescent band shows a red shift, from 440nm to 540nm. Among different QD solutions, we demonstrate an expansion of the emission range up to ~100 nm, thus improving their features as biomarkers. Moreover we show that optimized glutathione-capped quantum dots can directly bind GST blotted onto polyvinylidene difluoride (PVDF) membranes, and thus are suitable for the direct detection of GST fusion proteins.

Ključne riječi
quantum dots; fluorescence; glutathione; glutathione S-transferase fusion protein

Hrčak ID: 142871

URI
https://hrcak.srce.hr/142871

Posjeta: 1.993 *