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https://doi.org/10.5772/54650

Specific Binding of Alzheimer’s Aβ Peptide Fibrils to Single Walled Carbon Nanotubes

Steingrimur Stefansson ; Fuzbien Technology Institute, Rockville, MD, USA
Martha Knight ; CC Biotech Ltd, Rockville, MD, USA
Saeyoung Nate Ahn ; Fuzbien Technology Institute, Rockville, MD, USA

Puni tekst: engleski, pdf (582 KB) str. 2-11 preuzimanja: 369* citiraj
APA 6th Edition
Stefansson, S., Knight, M. i Ahn, S.N. (2012). Specific Binding of Alzheimer’s Aβ Peptide Fibrils to Single Walled Carbon Nanotubes. Nanomaterials and Nanotechnology, 2 (Godište 2012), 2-11. https://doi.org/10.5772/54650
MLA 8th Edition
Stefansson, Steingrimur, et al. "Specific Binding of Alzheimer’s Aβ Peptide Fibrils to Single Walled Carbon Nanotubes." Nanomaterials and Nanotechnology, vol. 2, br. Godište 2012, 2012, str. 2-11. https://doi.org/10.5772/54650. Citirano 19.09.2021.
Chicago 17th Edition
Stefansson, Steingrimur, Martha Knight i Saeyoung Nate Ahn. "Specific Binding of Alzheimer’s Aβ Peptide Fibrils to Single Walled Carbon Nanotubes." Nanomaterials and Nanotechnology 2, br. Godište 2012 (2012): 2-11. https://doi.org/10.5772/54650
Harvard
Stefansson, S., Knight, M., i Ahn, S.N. (2012). 'Specific Binding of Alzheimer’s Aβ Peptide Fibrils to Single Walled Carbon Nanotubes', Nanomaterials and Nanotechnology, 2(Godište 2012), str. 2-11. https://doi.org/10.5772/54650
Vancouver
Stefansson S, Knight M, Ahn SN. Specific Binding of Alzheimer’s Aβ Peptide Fibrils to Single Walled Carbon Nanotubes. Nanomaterials and Nanotechnology [Internet]. 2012 [pristupljeno 19.09.2021.];2(Godište 2012):2-11. https://doi.org/10.5772/54650
IEEE
S. Stefansson, M. Knight i S.N. Ahn, "Specific Binding of Alzheimer’s Aβ Peptide Fibrils to Single Walled Carbon Nanotubes", Nanomaterials and Nanotechnology, vol.2, br. Godište 2012, str. 2-11, 2012. [Online]. https://doi.org/10.5772/54650

Sažetak
Amyloids constitute a class of protein and protein fragments believed to be involved in the pathologies associated with Alzheimer’s, Parkinson’s and Creutzfeldt‐Jakob diseases. These proteins can self‐assemble into unique fibrillar structures that are resistant to normal protein degradation. Interesting recent developments in the study of amyloid fibrils demonstrate that they bind carbon allotropes. In this study, using single‐walled carbon nanotube field-effect transistors (SWCNT‐FETs), we show that the fibrillar form of Alzheimer’s amyloid β (1‐40) and (1‐42) peptides specifically bind non‐functionalized SWCNT in a saturable manner. Both peptides exhibited near identical binding curves with half‐maximal binding concentrations of approximately 12 µg/ml. Binding of the peptides to SWCNTs was diminished by including dimethyl sulphoxide (DMSO) at concentrations that inhibits fibril formation. Lastly, a monoclonal antibody (BAM‐10), which binds to the N‐terminal region of Alzheimer’s amyloid fibrils, recognizes the amyloid peptides adhering to SWCNTs in the absence of DMSO, but not in the presence of 75% DMSO. Taken together, these results suggest that the fibrillar form of the Alzheimer’s amyloid peptides are specifically binding to SWCNTs.

Ključne riječi
carbon nanotubes; Alzheimer's; amyloid peptides; transistor

Hrčak ID: 142875

URI
https://hrcak.srce.hr/142875

Posjeta: 577 *