Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/16.3.1622
Blood plasma proteins and protein fractions in roe deer Capreolus capreolus L.
Dorota CYGAN-SZCZEGIELNIAK
; Animal Biochemistry & Biotechnology Department; Chair of Biochemistry and Toxicology,University of Science and Technology, Mazowiecka Street 28, 85-084 Bydgoszcz, Poland
Karolina STASIAK
; Animal Biochemistry & Biotechnology Department; Chair of Biochemistry and Toxicology,University of Science and Technology, Mazowiecka Street 28, 85-084 Bydgoszcz, Poland
Bogdan JANICKI
; Animal Biochemistry & Biotechnology Department; Chair of Biochemistry and Toxicology,University of Science and Technology, Mazowiecka Street 28, 85-084 Bydgoszcz, Poland
Aleksandra ROŚLEWSKA
; Animal Biochemistry & Biotechnology Department; Chair of Biochemistry and Toxicology,University of Science and Technology, Mazowiecka Street 28, 85-084 Bydgoszcz, Poland
Magdalena STANEK
; Animal Biochemistry & Biotechnology Department; Chair of Biochemistry and Toxicology,University of Science and Technology, Mazowiecka Street 28, 85-084 Bydgoszcz, Poland
Sažetak
The aim of the research was to investigate some selected biochemical blood
parameters in roe deer (Capreolus capreolus L.). The experiment covered 15 from 2
to 3-year-old bucks from Kuyavian-Pomeranian Voivodeship. The animals were shot
by individual hunters on the shooting grounds during the hunting season of
2008/2009 (in the accordance with the Journal of Laws No 48).
The material for the research was blood plasma obtained after centrifuging full,
nonhemolyzed blood. The blood was collected from the zygomatic vein directly to the
test tubes with EDTA and transported in cooling conditions to the laboratory. After
transporting the samples of blood to a certified analytical laboratory, the following
elements of the obtained blood plasma were examined: ceruloplasmin – using
turbidimetric method; transferrin – using immunoturbimetric method; troponin- using a
third generation assay on an Elecsys; total protein, albumin, globulin – using
spectrophotometric method and total iron – using colorimetric method. The results
were statistically analyzed, i.e. the correlation between the parameters was
measured by means of Pearson’s correlation coefficient. The analysis of the results
revealed a number of statistically significant relations between the parameters under
the investigation, especially among the compounds directly responsible for
metabolism of iron and copper. A statistically important positive correlation was
observed between ceruloplasmin and ferritin (r = 0.563; P≤0.05) and a negative one
between transferrin and troponin (r = -0.609; P≤0.05). Moreover, the content of
transferrin – an iron-binding protein – was 0.17 g/l, while the concentration of iron
was 58 μmol/l. The content of ceruloplasmin – a protein responsible for metabolism
of copper – was very low (0.036 g/l). The level of proteins in the blood plasma of the
animals under the research was approximately 72 g/l, with the share of albumins
about 46%. The albumin-globulin ratio was 0.86.
Ključne riječi
biochemical blood parameters; game; roe deer
Hrčak ID:
145111
URI
Datum izdavanja:
15.9.2015.
Posjeta: 1.206 *