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https://doi.org/10.15255/CABEQ.2014.2022

Substrate Inhibition Growth Kinetics for Cutinase Producing Pseudomonas cepacia Using Tomato-peel Extracted Cutin

K. Dutta ; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India
V. Venkata Dasu ; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India
B. Mahanty ; Department of Environmental Engineering, INHA University, Incheon 402751, Republic of Korea
A. Anand Prabhu ; Biochemical Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology (IIT) Guwahati, Guwahati 781039, Assam, India


Puni tekst: engleski pdf 457 Kb

str. 437-445

preuzimanja: 1.087

citiraj


Sažetak

Using tomato-peel extracted cutin, an economically viable substrate, cutinase production by Pseudomonas cepacia was studied at different initial substrate concentrations (2–20 g L–1). The highest volumetric enzyme activity was observed at 10 g L–1 of cutin, which was inhibited at further higher concentrations. Various 3-, 4- and 5- parametric Monod-variant models were chosen to analyze the inhibition kinetics. The model parameters as well as goodness of fit were estimated using non-linear regression analysis. The
4- parameter Webb model was the best-fit model (R2 = 0.933), followed by the 3-parameter Andrews model (R2 = 0.92). Parameter sensitivity analysis revealed that the maximum specific growth rate was the most sensitive parameter for both the models, and the
Webb constant was the least sensitive. Finally, based on a strong evidence ratio 190.65 from Akaike’s information content criteria analysis as well as extra sum of square F test (P > 0.05), it was found that 3-parameter Andrews model gave the best fit.

Ključne riječi

cutinase; Pseudomonas cepacia; substrate inhibition model; Akaike’s information content criteria; parameter sensitivity

Hrčak ID:

147009

URI

https://hrcak.srce.hr/147009

Datum izdavanja:

14.10.2015.

Posjeta: 1.925 *