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https://doi.org/10.5562/cca2519

Interaction of Insulin and Polymer Surface Investigated by Surface-MALDI-TOF-Mass Spectrometry

Zahida Ademović   ORCID icon orcid.org/0000-0002-3780-1608 ; Faculty of Technology, University of Tuzla, Univerzitetska 8, 75000 Tuzla, Bosnia and Herzegovina
Jochen Salber ; Chirurgische Klinik und Poliklinik, BG Universitaetsklinikum Bergmannsheil, Ruhr-University Bochum, Germany
Doris Klee ; Department of Textile Chemistry and Macromolecular Chemistry, RWTH Aachen, Pauwelsstr. 8, 52074 Aachen, Germany

Puni tekst: engleski, pdf (823 KB) str. 213-219 preuzimanja: 591* citiraj
APA 6th Edition
Ademović, Z., Salber, J. i Klee, D. (2015). Interaction of Insulin and Polymer Surface Investigated by Surface-MALDI-TOF-Mass Spectrometry. Croatica Chemica Acta, 88 (3), 213-219. https://doi.org/10.5562/cca2519
MLA 8th Edition
Ademović, Zahida, et al. "Interaction of Insulin and Polymer Surface Investigated by Surface-MALDI-TOF-Mass Spectrometry." Croatica Chemica Acta, vol. 88, br. 3, 2015, str. 213-219. https://doi.org/10.5562/cca2519. Citirano 02.06.2020.
Chicago 17th Edition
Ademović, Zahida, Jochen Salber i Doris Klee. "Interaction of Insulin and Polymer Surface Investigated by Surface-MALDI-TOF-Mass Spectrometry." Croatica Chemica Acta 88, br. 3 (2015): 213-219. https://doi.org/10.5562/cca2519
Harvard
Ademović, Z., Salber, J., i Klee, D. (2015). 'Interaction of Insulin and Polymer Surface Investigated by Surface-MALDI-TOF-Mass Spectrometry', Croatica Chemica Acta, 88(3), str. 213-219. https://doi.org/10.5562/cca2519
Vancouver
Ademović Z, Salber J, Klee D. Interaction of Insulin and Polymer Surface Investigated by Surface-MALDI-TOF-Mass Spectrometry. Croatica Chemica Acta [Internet]. 2015 [pristupljeno 02.06.2020.];88(3):213-219. https://doi.org/10.5562/cca2519
IEEE
Z. Ademović, J. Salber i D. Klee, "Interaction of Insulin and Polymer Surface Investigated by Surface-MALDI-TOF-Mass Spectrometry", Croatica Chemica Acta, vol.88, br. 3, str. 213-219, 2015. [Online]. https://doi.org/10.5562/cca2519

Sažetak
Synthetic materials in contact with protein containing solution adsorb a considerable amount of proteins. The adsorption behaviour of zinc-free porcine insulin on the hydrophobic poly(vinylidene fluoride) (PVDF) surfaces before and after chemical vapour deposition (CVD) modification was directly analysed by matrix-assisted laser desorption ionisation-time-of-flight-mass spectroscopy in surface mode (surface-MALDI-TOF-MS). The MALDI mass spectra of Zn-free porcine insulin dissolved in carbonate buffer pH 8.3 after adsorption onto non-modified and modified PVDF-CVD surfaces contain peaks assigned to monomer ion peak as well as peaks that are results of degradation of Zn-free porcine insulin. The degradation is caused by structural changes taking place during adsorption of insulin onto hydrophobic surfaces and by subsequent laser induced desorption and ionisation process. Surface spectra of Zn-free porcine insulin dissolved in deionised water show only monomer ion peaks of porcine insulin without degradation product detected. Structure stability of Zn-free porcine insulin upon adsorption is influenced by hydrophobic interaction between insulin and the surface.

Ključne riječi
insulin; protein adsorption; biomaterials surface; MALDI-TOF-MS

Hrčak ID: 150524

URI
https://hrcak.srce.hr/150524

Posjeta: 892 *