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Predominance of CD4 Positive Bone Marrow Stromal Cells in Patients with Early Stage of Classic Hodgkin's Disease Mixed Cellularity Variant

Demetrio Tamiolakis
Ioannis Venizelos
Athanasia Kotini
Maria Lambropoulou
Theodoros Jivannakis
Anna Efthimiadou
Panagiotis Boglou
Nikolas Papadopoulos

Sažetak

The aim of the study was to determine the possible bone marrow involvement in patients with early stages of classic Hodgkin's disease mixed cellularity variant diagnosed by lymph node biopsy at initial presentation not responding to radiotherapy alone. The study cohort consisted of 20 patients (18 displaying B-cell genotype and two T-cell genotype) with stages I-II Hodgkin's disease according to Ann Arbor classification treated with radiotherapy alone, seven of them not responding to therapy. Southern blot hybridization using a specific EBV Bam H1W fragment probe showed the presence of EBV genomes in two patients. All 20 patients underwent iliac crest trephine biopsy and a panel of antibodies including CD45, CD20, CD4, CD8, CD45RO, CD56, CD30, ALK-1, CD-15, EMA, CD61, and CD68 were performed. A statistically significant predominance of CD45, CD45RO and CD4 positive stromal cells was found in seven patients that failed to respond to therapy (c2-test: p=0.021, p=0.019 and p=0.015, respectively). The predominance of CD4 positive cells in the bone marrow stroma might be suggestive of involvement by Hodgkin's disease in the early stage (I-II) patients (indicating upstaging) who fail to show remission on radiotherapy alone, and could explain the abnormal cytokine production, which may contribute to diminished T-cell immunity and inefficient antitumor responses despite a vast majority of infiltrating reactive immune cells.

Ključne riječi

Hodgkin's disease -pathology; Bone marrow - pathology; Hodgkin's disease - immunohistochemistry

Hrčak ID:

14546

URI

https://hrcak.srce.hr/14546

Datum izdavanja:

1.12.2004.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.374 *