Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2015.56.257
Identification of human remains from the Second World War mass graves uncovered in Bosnia and Herzegovina
Damir Marjanović
; International Burch University, Sarajevo, Bosnia and Herzegovina
Negra Hadžić Metjahi
; Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Jasmina Čakar
; Institute for Genetic Engineering and Biotechnology, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
Snježana Džijan
; Genos DNA Laboratory, Zagreb, Croatia
Vedrana Škaro
; Genos DNA Laboratory, Zagreb, Croatia
Petar Projić
; Genos DNA Laboratory, Zagreb, Croatia
Tomislav Madžar
; University of Zagreb, Medical School, Zagreb, Croatia
Eduard Rod
; St. Catherine Hospital, Zabok and Zagreb, Croatia
Dragan Primorac
; University of Zagreb, Medical School, Zagreb, Croatia
Sažetak
Aim To present the results obtained in the identification
of human remains from World War II found in two mass
graves in Ljubuški, Bosnia and Herzegovina.
Methods Samples from 10 skeletal remains were collected.
Teeth and femoral fragments were collected from
9 skeletons and only a femoral fragment from 1 skeleton.
DNA was isolated from bone and teeth samples using an
optimized phenol/chloroform DNA extraction procedure.
All samples required a pre-extraction decalcification with
EDTA and additional post-extraction DNA purification using
filter columns. Additionally, DNA from 12 reference
samples (buccal swabs from potential living relatives)
was extracted using the Qiagen DNA extraction method.
QuantifilerTM Human DNA Quantification Kit was used for
DNA quantification. PowerPlex ESI kit was used to simultaneously
amplify 15 autosomal short tandem repeat (STR)
loci, and PowerPlex Y23 was used to amplify 23 Y chromosomal
STR loci. Matching probabilities were estimated using
a standard statistical approach.
Results A total of 10 samples were processed, 9 teeth and
1 femoral fragment. Nine of 10 samples were profiled using
autosomal STR loci, which resulted in useful DNA profiles
for 9 skeletal remains. A comparison of established victims’
profiles against a reference sample database yielded 6
positive identifications.
Conclusion DNA analysis may efficiently contribute to
the identification of remains even seven decades after the
end of the World War II. The significant percentage of positively
identified remains (60%), even when the number of
the examined possible living relatives was relatively small
(only 12), proved the importance of cooperation with the
members of the local community, who helped to identify
the closest missing persons’ relatives and collect referent
samples from them.
Ključne riječi
Hrčak ID:
151293
URI
Datum izdavanja:
15.6.2015.
Posjeta: 1.354 *