Izvorni znanstveni članak
The relationship between Trichothecium roseum and Aspergillus parasiticus and the production of aflatoxins
S. Duraković
; Laboratory of Microbiology, Faculty of Food Science and Biotechnology, Zagreb, Croatia
B. Radić
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Z. Duraković
; Department of Internal Medicine, University Hospital Rebro, Zagreb, Croatia
Lj. Brečević
; Ruđer Bošković Institute, Zagreb, Croatia
O. Pospišl
; Laboratory of Microbiology, Faculty of Food Science and Biotechnology, Zagreb, Croatia
Sažetak
The relationship between mould biomass and the biosynthesis of aflatoxins B1 and G1 on solid substrates (whole and crushed maize grain) at temperatures from 15-35 °C and water content in the substrate of 20-36% has been investigated. The experiments have been carried out with the aflatoxigenous mould Aspergillus parasiticus NRRL 2999 in pure culture and in mixed culture respectively, the latter with the mould Trichothecium roseum. ZMFT 1226. Mould T. roseum does not produce aflatoxins and chromatographically similar compounds. The amount of biomass was estimated by measuring the chitin content, and the aflatoxin concentration by means of a fluorodensitometer. It has been established that the biosynthesis of the examined aflatoxins and their ratio primarily depend on the temperature of cultivation, rather than on the growth of the mycelium. The biomass of the mixed culture of A. parasiticus and T. roseum after seven weeks of cultivation reduces the amount of aflatoxin B1 by 20-38%, and the amount of aflatoxin G1 by 30-40%. The decrease of concentration of both toxins is more pronounced in the substrate with a higher initial water content and a higher temperature of cultivation.
Ključne riječi
Hrčak ID:
153861
URI
Datum izdavanja:
23.12.1986.
Posjeta: 862 *