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Izvorni znanstveni članak
https://doi.org/10.1515/botcro-2016-0003

Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut

Kaitheri Edathil Rachana ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India
Sudalaimuthu Asari Naganeeswaran ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India
Thayale Purayil Fayas ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India
Regi Jacob Thomas ; ICAR-Central Plantation Crops Research Institute (RS), Kayamkulam, Kerala, India
Muliyar Krishna Rajesh ; Division of Crop Improvement, ICAR-Central Plantation Crops Research Institute, Kasaragod 671124, Kerala, India

Puni tekst: engleski, pdf (4 MB) str. 1-10 preuzimanja: 851* citiraj
APA 6th Edition
Rachana, K.E., Naganeeswaran, S.A., Fayas, T.P., Thomas, R.J. i Rajesh, M.K. (2016). Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut. Acta Botanica Croatica, 75 (1), 1-10. https://doi.org/10.1515/botcro-2016-0003
MLA 8th Edition
Rachana, Kaitheri Edathil, et al. "Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut." Acta Botanica Croatica, vol. 75, br. 1, 2016, str. 1-10. https://doi.org/10.1515/botcro-2016-0003. Citirano 24.10.2021.
Chicago 17th Edition
Rachana, Kaitheri Edathil, Sudalaimuthu Asari Naganeeswaran, Thayale Purayil Fayas, Regi Jacob Thomas i Muliyar Krishna Rajesh. "Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut." Acta Botanica Croatica 75, br. 1 (2016): 1-10. https://doi.org/10.1515/botcro-2016-0003
Harvard
Rachana, K.E., et al. (2016). 'Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut', Acta Botanica Croatica, 75(1), str. 1-10. https://doi.org/10.1515/botcro-2016-0003
Vancouver
Rachana KE, Naganeeswaran SA, Fayas TP, Thomas RJ, Rajesh MK. Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut. Acta Botanica Croatica [Internet]. 2016 [pristupljeno 24.10.2021.];75(1):1-10. https://doi.org/10.1515/botcro-2016-0003
IEEE
K.E. Rachana, S.A. Naganeeswaran, T.P. Fayas, R.J. Thomas i M.K. Rajesh, "Cloning, characterization and expression analysis of NBS-LRR-type resistance gene analogues (RGAs) in coconut", Acta Botanica Croatica, vol.75, br. 1, str. 1-10, 2016. [Online]. https://doi.org/10.1515/botcro-2016-0003

Sažetak
Coconut palms are highly susceptible to diseases caused by different pathogens, and replanting with resistant varieties is the best way to manage them. Obtaining a collection of resistance gene analogues (RGAs) is an effective strategy to identify genomic regions linked to disease resistance. We have successfully used a comparative genomics approach to amplify putative RGAs from the coconut root (wilt) disease resistant cultivar Chowghat Green Dwarf (CGD) by using primers designed based on conserved motifs of the NBS-LRR domain of the date palm. The amplified sequences were cloned, sequenced and characterized. The coconut RGAs had high identity to monocot NBS-LRRs. A complete structural analysis and 3-D modeling of the NBS domain of coconut RGA was also undertaken. Real-time quantitative polymerase chain reaction analysis indicated that the isolated coconut NBS-LRR class RGAs was expressed more in root (wilt) disease resistant genotypes than in susceptible ones. This study would provide a base for future efforts to map disease resistant traits in coconut.

Ključne riječi
coconut; homology modeling; nucleotide binding site-leucine rich repeat type; real time PCR; resistant gene analogue

Hrčak ID: 155267

URI
https://hrcak.srce.hr/155267

Posjeta: 1.263 *