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Acta Pharmaceutica, Vol.66 No.3 Rujan 2016.

Kratko priopćenje
https://doi.org/10.1515/acph-2016-0027

Evaluation of pancreatin stability through enzyme activity determination

GLEYSSON DE PAULA TERRA ; Federal University of Alfenas – UNIFAL-MG, Institute of Chemistry, Laboratory of Analysis and Characterization of Pharmaceuticals, Alfenas, Minas Gerais, Brazil
MARCUS VINICIUS DE FARIAS RAMOS ; Federal University of Alfenas – UNIFAL-MG, Institute of Chemistry, Laboratory of Analysis and Characterization of Pharmaceuticals, Alfenas, Minas Gerais, Brazil
MARCELLO GARCIA TREVISAN ; Federal University of Alfenas – UNIFAL-MG, Institute of Chemistry, Laboratory of Analysis and Characterization of Pharmaceuticals, Alfenas, Minas Gerais, Brazil
JERUSA SIMONE GARCIA ; Federal University of Alfenas – UNIFAL-MG, Institute of Chemistry, Laboratory of Analysis and Characterization of Pharmaceuticals, Alfenas, Minas Gerais, Brazil

Puni tekst: engleski, pdf (364 KB) str. 423-431 preuzimanja: 958* citiraj
APA 6th Edition
DE PAULA TERRA, G., DE FARIAS RAMOS, M.V., TREVISAN, M.G. i GARCIA, J.S. (2016). Evaluation of pancreatin stability through enzyme activity determination. Acta Pharmaceutica, 66 (3), 423-431. https://doi.org/10.1515/acph-2016-0027
MLA 8th Edition
DE PAULA TERRA, GLEYSSON, et al. "Evaluation of pancreatin stability through enzyme activity determination." Acta Pharmaceutica, vol. 66, br. 3, 2016, str. 423-431. https://doi.org/10.1515/acph-2016-0027. Citirano 19.08.2018.
Chicago 17th Edition
DE PAULA TERRA, GLEYSSON, MARCUS VINICIUS DE FARIAS RAMOS, MARCELLO GARCIA TREVISAN i JERUSA SIMONE GARCIA. "Evaluation of pancreatin stability through enzyme activity determination." Acta Pharmaceutica 66, br. 3 (2016): 423-431. https://doi.org/10.1515/acph-2016-0027
Harvard
DE PAULA TERRA, G., et al. (2016). 'Evaluation of pancreatin stability through enzyme activity determination', Acta Pharmaceutica, 66(3), str. 423-431. doi: https://doi.org/10.1515/acph-2016-0027
Vancouver
DE PAULA TERRA G, DE FARIAS RAMOS MV, TREVISAN MG, GARCIA JS. Evaluation of pancreatin stability through enzyme activity determination. Acta Pharm. [Internet]. 30.09.2016. [pristupljeno 19.08.2018.];66(3):423-431. doi: https://doi.org/10.1515/acph-2016-0027
IEEE
G. DE PAULA TERRA, M.V. DE FARIAS RAMOS, M.G. TREVISAN i J.S. GARCIA, "Evaluation of pancreatin stability through enzyme activity determination", Acta Pharmaceutica, vol.66, br. 3, str. 423-431, Kolovoz 2018. [Online]. doi: https://doi.org/10.1515/acph-2016-0027

Sažetak
Pancreatin is a biotechnological product containing an enzyme complex, obtained from porcine pancreas, that is employed in treating pancreatic diseases. Experiments regarding the stability of the pharmaceutical formulation containing pancreatin were performed using standard binary mixtures with 6 excipients in a 1:1 ratio 1 (m/m) and a commercial formulation. To accomplish these goals, samples were stored for 1, 3 and 6 months at 40 ± 1 °C and 75 ± 5 % relative humidity (RH) and 40 ± 1 °C and 0 % RH. Stress testing was also performed. All samples were analyzed to evaluate the α-amylase, lipase and protease activities through UV/Vis spectrophotometry. The results revealed that the excipient proprieties and the storage conditions affected enzyme stability. Humidity was a strong influencing factor in the reduction of α-amylase and protease activities. Stress testing indicated that pH 9.0 and UV light did not induce substantial alterations in enzyme activity.

Ključne riječi
α-amylase; lipase; protease; stress testing; pancreatin; biopharmaceutical; UV/VIS spectrophotometry

Hrčak ID: 156872

URI
https://hrcak.srce.hr/156872

Posjeta: 1.051 *