Acta Botanica Croatica, Vol. 32 No. 1, 1973.
Izvorni znanstveni članak
Contribution to the Method of Etioplast Isolation
Živko Stanković
; Srbija
Sažetak
In the present study an attempt has been made to find more suitable plants for etioplast isolation and to obtain a higher percentage of intact isolated etioplasts.
Etioplasts have been isolated in 16 different plant species (broad bean, pea, bean, mustard, kale, radish, cucumber, pumpkin, sunflower, castor bean, soybean, crupina, barley, maize, oat and wheat). All isolated etioplasts have been carefully observed by microscopic methods, especially by phase-contrast microscopy. In 5 plant species (pea, radish, sunflower, pumpkin and maize) the etioplasts have been investigated in detail with electron microscope. Depending on plant species the isolation of etioplasts has been attempted in different media, designated as medium A, B, C and D. The basic medium — medium A — corresponds to a slightly modified medium as used by Jacobson (1968). The following results have been obtained:
Pea, broad bean, pumkin, sunflower, cucumber, crupina, radish, mustard, and all cereals have proved to be suitable plants for etioplast isolation, while bean, castor bean, soybean and kale have been shown to be unsuitable.
The highest number of isolated etioplasts (Table 1) can be obtained from the etiolated leaves of pea, and the lowest one from the cotyledon of sunflower.
The size distribution of the etioplasts varies in different plants (Hist. 1—5) and ranges from 2—2.5 ¡xm in radish, 2.5—3 ¡Jim in pea, pumpkin and maize, and 3—3,5 pm in sunflower.
The composition of the medium proved to be an important factor in the etioplast isolation. In most plants etioplasts can be successfully isolated in the medium A (see p. 71 and Figs. 1, 6); this medium, however, is less suitable for the isolation of etioplasts in broad bean (Fig. 3), radish (Fig. 8), and sunflower (Figs. 2, 7), and unsuitable in pea (Fig. 4).
Better results can be obtained in isolating etioplasts in pea (Figs. 5, 10), by adding a stabilizing component — 2°/o bovine serum albumin, (medium C), which indicates that this component stabilizes the structure of etioplasts.
Ključne riječi
Hrčak ID:
157251
URI
Datum izdavanja:
31.12.1973.
Posjeta: 918 *