Acta Botanica Croatica, Vol. 36 No. 1, 1977.
Izvorni znanstveni članak
Properties of Two Virus Isolates from Clovers in Croatia
Nysret Taraku
; Hrvatska
Nikola Juretić
; Hrvatska
Davor Miličić
; Hrvatska
Sažetak
Two viruses have been isolated from Trifolium repens and Trifolium pratense. The isolate found in T. repens (CMV-C) was identified as an isolate of cucumber mosaic virus (CMV). It was readily sap transmissible by sap and also by Myzus persicae Sulz., but was not soil-borne. Symptoms of test plants were similar to or identical with the symptoms common to CMV. CMV-C provoked leaf deformations and shoe-string symptoms of leaves on N. clevelandii, and Phaseolus vulgaris. Physical properties were well in concordance with those found for CMV. In serological investigations, performed by means of agar gel double diffusion test and absorption test in agar, CMV-C reacted positively with CMV antiserum. Plant-protection test have confirmed that CMV-C belongs to CMV.
The investigations of the isolate from T. pratense (BYMV-C) showed that it probably belonged to bean yellow mosaic virus (BYMV). The physical properties of BYMV-C correspond to the properties of BYMV isolates. The statement that BYMV-C represented an isolate of BYMV was supported by investigations of cell inclusions. Granular cytoplasmic inclusions in the form of x-bodies occured in the infected cells, but they were smaller than in the typical BYMV. The examined ultrathin sections of infected tissue revealed small aggregates of elongated virus particles, pin-wheel structures, and laminated aggregates. Another indication in favour of our conclusion that BYMV-C belongs to BYMV, is the positive serological reaction and moderately high heterologous titre U : 321 of BYMV antiserum (homologous titre 1 : 512) when tested with BYMV-C. It seems that BYMV-C represents a distinct strain of BYMV.
Ključne riječi
Hrčak ID:
158203
URI
Datum izdavanja:
31.12.1977.
Posjeta: 758 *