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Comparison of spectrophotometric, fluorimetric, high-performance liquid-chromatographic and radioimmunological methods for the determination of theophylline concentrations in biological material

F. Plavšić ; Laboratorij za kliničku farmakologiju, Zavod za kliničku farmakologiju, Kli¬nika za unutrašnje bolesti s poliklinikama Medicinskog fakulteta i Klinički bolnički centar, Zagreb


Puni tekst: hrvatski pdf 8.268 Kb

str. 173-183

preuzimanja: 358

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Sažetak

Spectrophotometric, fluorimetric, high-performance liquid chromatographic (HPLC) and radioimmunological (RIA) methods have been introduced for the determination of theophylline levels in the serum. The extraction on charcoal was used for drug isolation from biological fluids before spectrophotometric, fluorimetric and HPLC measurements. For the solid-phase RIA theophylline antibody was covalently bound to CNBr-activated Sepharose. For all introduced methods the inter- and intra-assay coefficients of variation were below 10 per cent in the therapeutic range of concentrations, and the sensitivity was below 1.0 mg. L-1. With the photometric methods high interferences of structurally similar drugs and other materials were observed. The correlations between different methods were good (r = 0.97 and 0.98).

Ključne riječi

Hrčak ID:

158563

URI

https://hrcak.srce.hr/158563

Datum izdavanja:

29.9.1982.

Podaci na drugim jezicima: hrvatski

Posjeta: 860 *