Acta Botanica Croatica, Vol. 46 No. 1, 1987.
Izvorni znanstveni članak
Factors Involved in Initiation of Somatic Embryogenesis in Cereal Tissue Culture
Zdendko Rengel
; Hrvatska
Sažetak
Recent data concerning somatic embryogenesis in cereal tissue culture are reviewed. A comparison between the culture of immature explants (embryo and inflorescences) and both mature embryo and parts of young seedlings is made. Somatic embryogenesis in the culture of immature explants proceeds from the explant cells which were embryogenic before explanting into culture, whereas a certain degree of redetermination of cells of more mature explants is necessary before induction of embryogenic callus in the culture of either mature embryo or parts of young seedlings. Embryogenic cells in the culture of more mature cereal explants do not seem to originate directly from explant cells, but the conversion of cells of preceding non-embryogenic callus to embryogenic ones is likely source of embryogenic callus. Physical isolation of a certain cell from its neighbourhood seems to be the most critical factor enabling somatic embryogenesis. Different stimuli, by which the escaping of the cell from control of the tissue as a whole can be achieved, are discussed (breaking of plasmodesmata, necrosis of surrounding cells, stress conditions and senescence).
Ključne riječi
Hrčak ID:
159298
URI
Datum izdavanja:
31.12.1987.
Posjeta: 1.216 *