Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2014.2130
Industrial Applications of Endoglucanase Obtained from Novel and Native Trichoderma atroviride
S. Sahin
; Suleyman Demirel University, Arts and Science Faculty, Department of Chemistry, 32260, Cunur, Isparta, Turkey
I. Ozmen
; Suleyman Demirel University, Arts and Science Faculty, Department of Chemistry, 32260, Cunur, Isparta, Turkey
H. Bıyık
; Biology Department, Faculty of Art and Science, Adnan Menderes University, Aydın, Turkey
Sažetak
An endo-β-1,4-glucanase (EG), produced under submerged fermentation by local isolate Trichoderma atroviride, was purified using ammonium sulfate precipitation, column and ion exchange chromatography with 55.16 fold and a specific activity of 30.9
EU mg–1. The studies of PAGE, SDS-PAGE and zymogram test have been carried out. The EG had optimum activity at pH 5.0 and 50 °C respectively. Using CMC as substrate, the enzyme showed maximum activity (Vmax) of 6.7 (μmol glucose min–1) mL–1 with its corresponding Km (Michaelis-Menten constant) value of 1.12 mg mL–1. While the EG activity was activated by NaCl, inhibited by MgCl2 and MgSO4. Otherwise, Tween 80, Triton X-100 and the total saponins known as biosurfactants enhanced the EG activity.
The obtained EG had low Km and high thermal stability. Additionally, usability of the EG in biotechnological application was investigated. The results showed that the EG had potentially sufficient effects on denim fabric and pretreated lignocellulosic wastes.
Ključne riječi
endo-β-1,4-glucanase; purification; characterization; biosurfactant; saponin; Trichoderma atroviride
Hrčak ID:
161835
URI
Datum izdavanja:
14.7.2016.
Posjeta: 1.555 *