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https://doi.org/10.1515/botcro-2016-0030

An eco-physiological and biotechnological approach to conservation of the world-wide rare and endangered aquatic liverwort Riella helicophylla (Bory et Mont.) Mont.

Marko S. Sabovljević orcid.org/0000-0001-5809-0406 ; Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
José Gabriel Segarra-Moragues ; Departamento de Biología Vegetal, Facultad de Ciencias Biológicas, Universitat de València. Avda. Dr. Moliner, 50, E-46100, Burjassot (Valencia), Spain
Felisa Puche ; Departamento de Botánica, Facultad de Ciencias Biológicas, Universitat de València. Avda. Dr. Moliner, 50, E-46100, Burjassot (Valencia), Spain
Milorad Vujičić ; Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia
Annalena Cogoni ; Dipartimento di Scienze della Vita e dell’Ambiente – Sezione Botanica ed Orto Botanico,Università di Cagliari, Italy
Aneta Sabovljević ; Institute of Botany and Botanical Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia

Sažetak

The rare aquatic liverwort Riella helicophylla (Bory et Mont.) Mont., inhabitant of temporary shallow ponds around the Mediterranean basin, is considered threatened throughout its distribution range. In addition, little is known of its biology and ecology or of its role in such an important ecosystem where environmental conditions vary yearly in unpredictable ways. In these variable habitats, due to the seasonal fluctuation of water levels, there is no guarantee of yearly spore input into the spore bank. Spore germination rate and the effects of different culture media in an axenic culture establishment, as well as propagation procedures of R. helicophylla, were tested. New insights into the ecology and biology of R. helicophylla are given. Spore dormancy is documented, and the protocols for the in vitro culture establishment, propagation and acclimatization of this liverwort are developed. Dry storage at 20 ± 2 °C for about three months broke the dormancy of spores, which subsequently germinated in a high percentage (over 90%). A two phase (solid and liquid) culture media system was developed for the purpose of achieving fully developed gametophytes. The liquid phase contained electrolytes simulating brackish water.

Ključne riječi

conservation; ecology; propagation; Riella; temporary ponds

Hrčak ID:

166899

URI

https://hrcak.srce.hr/166899

Datum izdavanja:

1.10.2016.

Posjeta: 1.134 *