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https://doi.org/10.2478/v10004-007-0027-1

Application of Recombinant DNA Methods for Production of Cholinesterases as Organophosphate Antidotes and Detectors

Palmer Taylor
Elsa Reiner
Zrinka Kovarik
Zoran Radić


Puni tekst: engleski pdf 150 Kb

str. 339-345

preuzimanja: 1.128

citiraj


Sažetak

To develop new avenues for synthesizing novel antidotes for organophosphate poisoning and for detection of the organophosphates, we have turned to recombinant DNA methods to synthesize cholinesterases with unusual properties. For antidotal therapy we describe mutations of the native mouse and human enzymes that allow for enhanced rates of oxime reactivation. Such enzymes, when localized in the circulation, would enable the circulating cholinesterase to become a catalytic rather than simply a stoichiometric scavenger. Hence, “oxime-assisted catalysis” provides a means for scavenging the organophosphates in the circulation thereby minimizing their tissue penetration and toxicity. Accordingly, the oxime antidote or prophylactic agent has a dual action within the circulation and at the tissue level. Second, through a novel chemistry, termed freeze-frame, click chemistry, we have used organophosphate conjugates of acetylcholinesterase as templates for the synthesis of novel nucleophilic reactivating agents. Finally, acetylcholinesterase can be modified through cysteine substitution mutagenesis and attachment of fluorophores at the substitution positions. When linked at certain locations in the molecule, the attached fluorophore is sensitive to organophosphate conjugation with acetylcholinesterase, and thus the very target of insecticide or nerve agent action becomes a detection molecule for organophosphate exposure.

Ključne riječi

acetylcholinesterase; fluorescent acetylcholinesterase; organophosphate inhibitors; oxime antidotes; oxime-assisted catalysis; reactivation kinetics; remote detection; in situ - click chemistry; template-guided drug design

Hrčak ID:

16533

URI

https://hrcak.srce.hr/16533

Datum izdavanja:

26.9.2007.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.392 *